Crime Scene Lab Report

The purpose of this lab was to analyze DNA from the crime scene at the school from three suspects through the process of using gel electrophoresis. Methods used to preform this lab was inserting DNA samples into wells while using micropipettes. Timing and measuring were methods used during this lab as well. Also, DNA fingerprinting was used to figure which of the three suspects broke into the school that night. In this experiment, blood samples were taken from all three suspects to be compared with the definite suspect. The group placed DNA samples into wells using micropipettes. Once the DNA was loaded the power source was turned on, and the electric current flowed for a total of 40 minutes. After, the results were stained, then the stain

One of the human errors in the lab was the group was destaining the gel in the sink to rapidly and the gel broke in half cutting off some of the important data. This error could simply be fixed by being more gentle with the data. Also, during the begging of the lab while filling the wells with gel the group did not put the same amount in each. This error could be fixed by simply taking time filling the wells and making sure no more gel is in the micropippette before moving on to the next well. Finally, another human error consisted of the power source getting shut off when our gel was not in it for a total of 20 minutes. The error could be fixed by getting more outlets in the room so the power so the power source was not shut off to early. The major experimental error was the groups were not able to fully distain the gel since the class was over after short amount of time. To fix this experimental error starting the lab sooner and planning more efficiently would allow for enough time to distain the gel. In addition, the buffer after used for one experiment was placed back into the original bottle and used for multiple other experiment what if the buffer had been overused and tampered other experiments would get inconclusive results. An easy way to fix this experimental error is getting little bottles of buffer and throwing them away each time rather then using the same one each time. Thus, if these experimental and human errors were fixed then the outcomes would be more accurate and the interpretation of the data would be