Capillary Electrophoresis Synthesis Lab Report

One significant advantage of capillary electrophoresis (CE) is the separation of a broad range of analytes at the same moment. Affinity Capillary Electrophoresis (ACE) is a technique used in order to separate substances which participate either in specific or in non-specific affinity interactions during the electrophoresis process, by using a capillary electrophoresis format. The molecules can be free in solution or they can be immobilized to a solid support (Heegaard, Nilsson and Guzman, 1998).
There are three modes of affinity capillary electrophoresis, namely the non-equilibrium electrophoresis of equilibrated sample mixtures, the dynamic equilibrium affinity electrophoresis and the affinity-based capillary electrophoresis (CE) or capillary electrochromatography (CEC) separations on immobilized selectors. In the first one, the receptor and the ligand are in the sample and the electrophoresis separation buffer is empty of both receptor and ligand. A portion of the total sample solution is

The determination of various conformations contributed to long lifetimes, and also the wide range of protein folding is the main reason for acceptable changes in the shape/size, charge distribution, or exposure of interacting domains to change electrophoretic velocities (Heegaard, Jorgensen, Rozlosnik, Corlin, Pedersen, Tempesta and Roepstorff, 2005).
Furthermore, microelectrophoretic methods have been used for the investigation of enzyme-substrate reactions which can be characterized as noncovalent molecular interactions. Both the screening and the quantitative online characterization of substrate formation and the inhibitor action can be conducted by electrophoretical mixing. Moreover, the electrophoretically mediated microanalysis (EMMA) setup has been used for the separation of zones of reactants and products (Burns and May,