Title: Preparation of thin blood smear.
Aim and objective: To produce blood smear and stain by using the wrights stain
Introduction:
A blood smear is a diagnostic test used to look for unusualness within the blood. The blood film gifts the opportunity to view the components of blood under a microscope for unusual shapes or sizes. There are three main cells within the blood that the test can focus such as red blood cell, white blood cells, and platelets.
Red blood cell or erythrocytes carry
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Using your right hand, the smooth clean edge of a spreader on the specimen slide is placed in front of the blood drop.
4. The spreader slide is held at a 30°, and the drop of blood is drawn back
5. The blood is spread almost to the edges
6. The spread is pushed forward with one light, smooth, and fluid motion. A thin of blood in the shape of a bullet or tongue with a feathered edge will remain on the slide.
7. The frosted edge is labelled with patient name, identity card, and date.
8. The blood film is allowed to air-dry completely before staining.
Preparation of Thin Blood Smear:
1. A small drop of venous blood is placed on a glass of microscope slide, using a disposable pipette. Used a wooden applicator stick for this purpose
2. A spreader slide is positioned at an angle and drawn slowly toward the drop of blood.
3. Brought the spreader slide in contact with the drop of blood and drawn it away. The edge of the spreader slide is noted.
4. The spreader slide is pulled out further makes a thin layer of blood.
5. The blood smear is nearly completed
6. Result ended. A glass slides with a well-formed blood film. After dried for about 10 minutes, the slide is stained manually or an automated slide stain is placed.
Staining
Peschel, O., Kunz, S. N., Rothschild, M. A., & Mützel, E. (2011). Blood stain pattern analysis. Forensic science, medicine, and pathology, 7(3), 257-270. http://link.springer.com/article/10.1007/s12024-010-9198-1
The idea is to get a sort of background story from the random blood distribution. The different types of blood can tell us the method an assailant uses, such as passive blood stains. These stains are made from the drops of a weapon due to gravity. For instance, if an attacker walked away from stabbing someone, the weapon would leave a series of drops.... ... middle of paper ...
The procedure of the test is simple hence patients will then be asked to remove their clothes and wrap and cover with a paper cloth around the waist before the Pap test. Patients will then be asked to lie back on the examine table with their legs spread open .Knees have to be bent and feet sitting on rest position. The clinician or gynecologist will insert the lubricated instrument called speculum into the patient’s vagina in a way that will hold open to see the cervix clearly. The clinician will examine to see if there is any visible problem and will gently scrape the surface of the cervix with the help of wooden scraper or spatula to obtain the sample of the cells. The liquid sample will then be smeared on a glass slides and will be brought to a laboratory to analyze where microscope slides will be ready for the test. The clinicians will have to repeat the test if the result is abnormal .Cells abnormalities are divided as follows ASC atypical squamous cells ,AGC,AIS,LSIL.
The largest component of the blood is the Plasma, a liquid substance made up of 91.5% water and 8.5% solutes that is responsible for over half the blood’s total volume. This liquid portion of the blood is vital for maintaining blood pressure within the body that helps capillary exchange. (Tortura 691-692). Cases of hemorrhagic or hypovolemic shock, a severe reduction in plasma is what triggers the body’s feedback systems, and compensation for fluid loss will stem from pulling of water from other
Many people throughout the world visit the United States every year to receive medical treatment. This is due to our excellent pharmaceutical industry as it spends million of dollars and many hours of research to come up with what we can only describe as “miracle” drugs and treatments. Part of the success of many of these medications is because the pharmaceutical industry is highly regulated by policies that protect the public from accessing drugs that have not been fully tested and found to be “safe”. However, this was not the case until the late 1990s and early 2000s. One time in history that highly influenced the strict regulations we currently have was the nationwide contamination of patients through blood transfusion or by consuming medications
strips was put into each tube and then timed with a stop clock and the
dragging of the tissues under the skin. Follow the rule “the deeper you go, the slower you go”.
· A sterile cloth is then put over your legs and abdomen and a sterile
...f the clamps on the tubing to allow the IV solution to run freely. Slowly, decrease the flow of the solution to the appropriate rate as ordered by the physician. Using a small gauze pad, wipe away any excess blood or fluid on the surface of the skin. Then, using the pre-torn pieces of tape, secure the catheter hub and the IV tubing to the patient’s skin. Take extra caution not to kink the tubing. Once everything is secured, recheck the IV solution’s flow and then attend to the rest of your patients needs.
Complete Blood Count with Differential is one of the most commonly ordered tests for routine check-ups and/or physicals. A complete blood count with differential measures the levels of red blood cells, white blood cells, platelet levels, hemoglobin and hematocrit. Most of the time it is ordered as a screening test to check for anemia or infection. The Complete Blood Count with Differential is used to aid in diagnosing and treating a large number of conditions (Lockwood, 2013).
Blood stains are one type of evidence that can be found at a crime scene. Blood that is still in the liquid form should be picked up on a gauze pad. Once the blood is dried thoroughly it should be refrigerated and sent to the Laboratory (Andrus et al., n.d., para. 1). If the blood stain is found dried on clothing, the officer should wrap the piece of clothing in clean paper and place it in a sealed and labeled container. An object with dried blood stains needs to be sent to the Laboratory if it is small enough. If the object is too large to send, then using a clean knife the stain needs to be scraped onto a clean piece of paper, which then can be folded and placed in an envelope (Andrus et al., n.d., para. 2). When collecting autopsy blood samples, the officer should request that the pathologist obtain the sample directly from the heart and place it in a yellow or purple stoppered vacutainer. If the victim is still alive but in serious need of a blood transfusion, then the pre-transfusion blood sample needs to be obtained promptly before the hospital discards it (Andrus et al., n.d., para. 4). It is important for the Laboratory to receive all blood samples within 48 ho...
Then, put your mouth on the area and suck the skin. The blood will come to the opposite side of the skin and it will create a red mark. It will stay there
platelets on a slide, you would need to have the specimen recollected because of a clot, or
I will be investigating Human Blood as my specific tissue and giving an overview on the location, characteristics, and the benefits it has to the human body. Blood is extracellular matrix that is consists of plasma, red blood cells, platelets, and white blood cells. Blood is located within the capillaries/veins/arteries of the human body, which are blood vessels that run through the entire body. These blood vessels allow the blood to flow smoothly and quickly from the heart to distinct parts of the human body. The unique parts of human blood all work together for a purpose: the Red Blood Cells(erythrocytes) transports oxygen throughout the body, White Blood Cells(leukocytes) play a part in the bodies immune system, Platelets(thrombocytes) assist in creating scabs,