Blackberry Breeders have Implement Morphological Marker-assisted Selection

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Blackberry breeders have implement morphological marker-assisted selection for thornlessness using Rubus L, which trigger development of molecular marker for blackberry cultivation. However, no genetic map and molecular marker exist for cultivation purpose. Therefore, the purpose of this study is to develop genetic map, molecular markers, or first blackberry expressed sequence tag (EST) library. The newly release of two blackberry (Rubus L) cultivars with new trait, primocane fruiting has the potential to expand the industry by combining primocane fruiting with useful trait such as thornlessness.

To compensate lacking of molecular linkage map and molecular marker, simple sequence repeat (SSR) has been generated from strawberry EST sequences from Genbank. Strawberry is a related species with blackberry by possessing the same sub-family, Rosoideae. The primer pair (SSR) has detected 68% and 43% polymorphisms for sequences with six or more and less than six repeats respectively. Thus, it was expected that useful SSR can be generated from blackberry and sufficient EST for construction of genetic map.

Blackberry cultivars that produce fruit in summer is called floricanes, canes that go dormant and come back in second spring. Meanwhile, primocanes fruiting cultivars produce fruit together with floricanes and subsequently produce a smaller second batch in late summer and early fall. This can prolonged period of harvesting for farmer, marketer, and consumers.

Methodology

Development of EST was started by choosing blackberry cultivars (Merton Thornless). Reason of choosing is because it is the progenitor. Firstly, the leaves of blackberry is been harvested at farm field. The leaves were wrapped in aluminium foil and th...

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...ted genes were ordered by relative abundance, as determined by the number of ESTs in the contigs, and was grouped by their putative functional catagories. Of the 288 EST analysed, the largest number are associated with energy has a sequence similar with Ribulose 1,5-biophosphate carboxylase (RUBISCO) The second greatest abundance was cell structure that associated with chlorophyll a/b binding protein followed by third largest categories which is disease and defense that associated with stress response. With this, unknown gene function can be discover.

Works Cited

Lewers, K. S., Saski, C. A., Cuthbertson, B. J., Henry, D. C., Staton, M. E., … Tomkins, J. P. (2008). A blackberry (Rubus L.) expressed sequence tag library for the development of simple sequence repeat markers. BMC Plant Biology, 8(69). Retrieved from http://www.biomedcentral.com/1471-2229/8/69.

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