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Specificity of salivary amylase
Specificity of salivary amylase
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In the experiment “Digestion of Carbohydrates by Salivary Amylase” this dealt with a test where amylase was observed from a saliva sample and starch mix. Salivary amylase is a digestive enzyme that catalyzes the hydrolysis of starch of starch into simpler compounds (Saunders 1). The main function for salivary amylase is to break down starch. When it comes to starches, they are known to be complex carbohydrates. In order to see whether or not the amylase broke down the starch, you can add iodine to the saliva sample and starch mix. When the iodine is added and if the solution remains purple, the starch was not broken down. Although, if the solution turned into a yellowish color the starch was in fact broken down. What this shows is the amylase …show more content…
First, you obtain a sample of saliva and do so by putting a couple of ounces of water in your mouth, swishing it around, and then spitting it into a small cup. Then, you label the two test tubes with your initials on them and labeling them by number one and number two. Then, you add ten drops of starch solution and ten drops of water to the first test tube. For the second test tube you do the same as well. After this is done, you then place both test tubes in the water bath for thirty minutes. When you place the test tubes in there label the two wells of the spot place one and two with the wax pencil provided. Finally, add one drop of the iodine solution to each well. Once this is all done, you can then record and analyze your …show more content…
First, label the three test tubes number one, two, and three. For each test tube, you need to add three mL of vegetable oil. Now for test tubes numbers one and two, you need to add five mL of water. Only for test tube number three add two mL of lipase enzyme. For only test tubes number two and three, add a pinch of bile salts. Once this is all done, mix the tubes by flicking the bottoms. Afterwards, place all three tubes in a water bath for only thirty minutes. Once this is all completed, you can then record and analyze the
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
The procedure of the lab on day one was to get a ring stand and clamp, then put the substance in the test tube. Then put the test tube in the clamp and then get a Bunsen burner. After that put the Bunsen burner underneath the test tube to heat it. The procedure of the lab for day two was almost exactly the same, except the substances that were used were different. The
Equipment list: Test tubes were used to hold the milk, the lipase and the milk and lipase solutions. Test tube racks were used to hold the test tubes
== Amylase is an enzyme found in our bodies, which digest starch into
I blanked it with 2 cm³ water, 1 cm³ amylase and 3 drops of iodine.
The temperature of amylase The temperature of starch Room temperature Concentration Ph values The variable I will be changing is the volume of amylase. Safety: The sand is To make sure I carry out this experiment safely I will make sure I wear a pair of goggles. I will ensure I keep my stool under the table and all
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
Investigating the Effect of Enzyme Concentration on the Hydrolysis of Starch with Amylase Aim: Investigate the effect of enzyme concentration on the rate of an enzyme-controlled reaction. Using amylase and starch as my example. Introduction: I am investigating the effect of the concentration of the enzyme, amylase on the time taken for the enzyme to fully breakdown the substrate, starch to a sugar solution. The varied variable will be the concentration and all other variables are going to be fixed. The different concentrations will be: 0.5% 0.75% 1.0% 1.5% 2% An enzyme is a class of protein, which acts as a biological catalyst to speed up the rate of reaction with its substrates.
The origination of the digestive process occurs prior to the turkey sandwiches introduction in the mouth. The eyes and the nose are the beginning of the digestive process by seeing the food and smelling; this causes a response in this in the brain by nerves stimuli creating a visual and chemical sense. The visual stimulation causes the mouth to activate the salivary glands in preparation for the food. As we commencement to the consumption of a turkey sandwich, consisting of bread, turkey, lettuce, and cheese. Our eyes would see the sandwich, our nose would smell the aroma making our mouth water in anticipation of the food. With the mouthwatering the chemical digestion process has already begun with this secretion of saliva that contains the
Prepare .05 to .075 g of crude lipid by dissolving it in hexane. Add to the silica gel slurry in the column.
What happens to food once it is ingested? Where does it go? How is it broken down into smaller pieces? The digestive process is very complex, but simple to understand. It involves several steps that include from being chewed inside the mouth, to landing in the stomach for more breakdown, traveling through the intestines, and finally exiting the body.
To investigate the amount of oxygen foam (cm) produced by the enzyme catalase when it breaks down hydrogen peroxide in the animal (liver, milk, honey) and plant cells(potato, purple cabbage) into oxygen and water
Create wells: put a comb template in the middle of the tray; wait until the mixture becomes solid. After, remove the comb standing straight. 4. Remove rubber ends: transfer the gel tray into the horizontal electrophoresis and fill it with the concentrated electrophoresis buffer. 5. Materials and methods: Experiment: 1st, prepared milk samples should be already done by the teacher.
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =
solutions and add them to a test tube rack. I will then cut up a