Serial Dilution Lab Report

Table of Contents
Experiment : 2
Objective : 2
Procedure : 2
Observations: 2
A. Ca2+ stock and substock Preparation: 2
B. Aliquot method : Ca2+ external calibration solution 3
D. Aliquot versus serial dilution method 9
Discussion 11
References : 11

List of Tables and figures:
Table 1 Data for Aliquot method 2
Table 2 Data for Serial dilution method 2
Table 3 Concentration Data for Aliquot method 4
Table 4 Experimental andf actual concentration for aliquot method 4
Table 5 Concentration data for serial dilution method 7
Table 6 Experimental and actual concentration for serial dilution method 7 figure 1 Aliquot dilution method …………………………………………………………………………………………………………....5 figure 2 Seral dilution method…………………………………………………………………………………………………………………8

1.056 0.943
2. 2.067 1.887
3. 3.051 2.831
4. 4.049 3.774
5. 5.040 4.718

Add 1%v/v HCl up to 250ml Stock slotion Transfer in 471.8 ppm

10ml from stock solution up to 100 ml Sub stock solution

1 ml 2 ml 3 ml 4 ml 5 ml

l 50 ml 50 ml 50 ml 50 ml 0.943 ppm 1.887 ppm 2.831 ppm 3.774ppm 4.718 ppm
Figure 1. Aliquot method dilution method

Construct a manual calibration plot of experimental absorbance versus actual Ca2+ concentration (pink line) using finely lined graph paper. Include proper title and figure #. Label the axes. Using the same graph paper plot experimental absorbance versus experimental Ca2+ concentration (green line). Include a legend to identify both

1.817 1.698
4. 1.025 0.849
5. 0.653 0.509

10. Stock solution

250 ml 471.8 ppm take 10 ml of solution and dilute up to 100 ml Sub stock solution

47.18 ppm Take 5 ml of solution and dilute Up to 50 ml

50 ml 4.718 ppm 50 ml 0.509 ppm 30ml 30 ml

30 ml 25 ml 50 ml 50 ml 50 ml 2.830 ppm 1.698ppm 0.849 ppm
Figure 2 : Serial dilution