Investigating the Link Between Wavelength of Light and Rate of Photosynthesis
PROBLEM =======
I have been asked to investigate the link between wavelength of light and rate of photosynthesis.
HYPOTHESIS ==========
I predict that the order of best absorption in a plat to produce more bubbles will be blue, yellow, orange, red and finally green. I predict this because blue has the shortest wavelength which produces the most energy and there is slightly higher absorption in the blue region by the plant. The red has the largest wavelength in the visible spectrum which produces the least energy. The reason why green is at the bottom of the list of absorption in a plant is because
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They absorb light energy and enable it to be converted into chemical energy which is used by the plants to make glucose and oxygen from carbon dioxide and water. Plants appear to be different colours because of the dominant pigments they contain. These pigments absorb some colours of light and reflect others, for example, the green chlorophylls absorb light from the blue-violet and the red regions of the visible spectrum and reflect green light. This is why plants which contain mostly chlorophylls appear green. Other pigments found in green plants, the yellow, orange and red carotenoids which absorb light only from the blue-violet region of the spectrum, are mostly masked by the more dominant chlorophyll.
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We can see how different wavelengths of light affect photosynthesis by looking at action spectra. An action spectrum relates the rate of photosynthesis to the wavelength of light being received by a plant. For green plants, including algae, the action spectrum shows that
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Photosynthetic activity is lowest in green light since green light is hardly absorbed at all by these pigments. The relative absorption of light of different wavelengths by pigments can be shown in absorption spectra. Action and absorption spectra correspond quite closely. Wavelengths of light which are more readily absorbed by photosynthetic pigments cause higher levels of photosynthesis.
Some plants live in conditions where the spectral quality of light may be different to that received by plants living on the land. Algae which live in surface waters tend to be green and contain more or less the same pigments as land plants since they exist under similar light conditions. Algae living lower in the water receive more blue light than red because red light has a relatively long wavelength and cannot penetrate water as well as blue light which has a shorter wavelength and more energy than red light.
Brown algae, which may be found deeper in the water than green algae, have combinations of pigments which enable them to
Photosynthetic pigments work by absorbing different wavelengths of light and reflecting others. These pigments are divided into two categories primary (chlorophyll) and accessory (carotenoids) pigments. Chlorophyll is then divided into three forms a, b, and c (Campbell, 1996). Chlorophyll a is the primary pigment used during photosynthesis (Campbell, 1996). This pigment is the only one that can directly participate in light reactions (Campbell, 1996). Chlorophyll a absorbs the wavelengths of 600 to 700nm (red and orange) along with 400 to 500nm (blue and violet) and reflects green wavelengths (Lewis, 2004). Chlorophyll b has only a slight difference in its structure that causes it to have a different absorption spectra (Campbell, 2004). The carotenoid involved with spinach leaf photosynthesis absorbs the wavelengths of 460 to 550nm (Lewis, 2004). The pigments are carotene and its oxidized derivative xanthophylls (Nishio, 2000). A wavelength is determined by measuring from the crest of one wave to the crest of the next wave. All the wavelengths possible are...
at the small end of the axis to see a more reliable pattern. I could
This shows that there could be three variables in this experiment, carbon dioxide, water and light energy. So in our case the variable light energy (light intensity) will be used. The equation also shows that if there is more light energy then more glucose and oxygen will be produced.
Investigation Into the Colour of Light Needed to Start a Photosynthesis Reaction Diagrams [IMAGE] Method The apparatus for this experiment will be setup as shown in the diagram, for all the experiments the coloured filter will be 5cm away from the white light bulb, which is a 60watt bulb, and the beaker will be 20cm away from the edge of the coloured filter. Just before the experiment takes place we will place the pond weed (which has been in darkness for at least 24 hours to stop it any photosynthesis) into the beaker. I will then place the funnel over the pond weed and place the test-tube into the beaker (like the diagram above) I will then fill the beaker up with cold tap water till it covers the bottom of the test tube (450ml).
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
Autotrophs, can build organic compounds from simple molecules such as water and carbon dioxide and their type of feeding is called autotrophic nutrition. While they are building complex molecules, they need large amounts of energy. They are divided into two groups according to their source of energy: chemoautotrophs and photoautotrophs. Chemoautotrophs can synthesize organic compounds from CO₂ AND H₂O by using inorganic oxidation energy and they do not require sunlight. However, photoautotrophs, including green plants, produce sugar and O₂ from CO₂ and H₂O by using sunlight. The green pigment which absorbs the light is called chlorophyll and this process is called photosynthesis.
During the following lab, an aquatic plant was covered with a funnel and was placed underwater inside a beaker, with a graduated cylinder (submerged in water) was placed over the neck of the funnel. This lab tested out which source of light which is the independent variable would allow the aquatic plant to produce the most bubbles (dependent variable) and photosynthesize the fastest. This is clearly displayed because when photosynthesis is produced underwater it appears in the form of bubbles inside the water. These bubbles will travel up through the funnel and displace the water in the graduated cylinder. The dependent variable was tested by placing the four, 5cm aquatic plants in the beakers directly in front of the sources of light and observing how many bubbles were produced within the period of 10 minutes and how much water was displaced by the oxygen bubbles. The beaker in front of the light source with the most bubbles produced and with the most displaced water performed photosynthesis faster than all the other light sources. The sources of light used for this lab were sunlight, florescent, red, and yellow lamp lights, and
An Analysis and Evaluation of Data from Photosynthesis Experiments Graph analysis This is my analysis for the investigation in to the affect of light intensity on the rate of photosynthesis to the Canadian pondweed, elodea. In the results the pattern is that when the light intensity is higher the readings are generally higher. On the graph the less the light intensity the lower the gradient of the curve. the equation for the photosynthesis process is; CO2 + 2H2O + Light Energy = =
To make the test fair I will use the same amount of water and the leaf
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
* Count the number of bubbles seen in 1 minute which is a way of
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Wavelength on Photosynthesis Rate Aim: To be able to To investigate how different wavelengths (colors) of light affect the photosynthetic rate of the synthetic. I will use a pant that is a pond weed called elodea. I will measure the rate of photosynthesis by measuring the amount of o2 given off in bubbles per minute from the elodea. I will do this by placing the Elodea in a test tube with sodium hydrogen. carbonate then I will vary the light wavelength (color) using colored.
The structure of chlorophyll involves a hydrophobic tail embedded in the thylakoid membrane which repels water and a porphyrin ring which is a ring of four pyrrols (C4H5N) surrounding a metal ion which absorbs the incoming light energy, in the case of chlorophyll the metal ion is magnesium (Mg2+.) The electrons within the porphyrin ring are delocalised so the molecule has the potential to easily and quickly lose and gain electrons making the structure of chlorophyll ideal for photosynthesis. Chlorophyll is the most abundant photosynthetic pigment, absorbing red and blue wavelengths and reflecting green wavelengths, meaning plants containing chlorophyll appear green. There are many types of chlorophyll, including chlorophyll a, b, c1, c2, d and f. Chlorophyll a is present in all photosynthetic organisms and is the most common pigment with the molecular formula C55H72MgN4O5. Chlorophyll b is found in plants with the molecular formula C55H70MgN4O6, it is less abundant than chlorophyll a. Chlorophyll a and b are often found together as they increase the wavelengths of light absorbed. Chlorophyll c1 (C35H30O5N4Mg) and c2 (C35H28O5N4Mg) are found in algae, they are accessory pigments and have a brown colour. Chlorophyll c is able to absorb yellow and green light (500-600nm) that chlorophyll a