Making a Wet Mount Slide
Purpose: The purpose of this lab experiment is to learn how to make a wet mount slide and observe it under a microscope.
Materials: The materials used in the lab experiment were…
1. Microscope
2. Microscope slide
3. Coverslip
4. Newspaper
5. Scissors
6. Medicine dropper
7. Water
8. Forceps
Procedure: The procedure followed in this experiment were…
1. Cut a small "d" from the newspaper and place it in the center of a clean microscope slide so that it is in normal reading position.
2. To make a wet-mount slide use the eye dropper and place a small drop of water over the specimen "letter "d") to be observed.
3. Place one slide of a clean coverslip at the edge of the drop of water at a 45-degree angle. Using the forceps carefully lower the coverslip over the letter "d" and drop of water. Try not to trap air bubbles under the coverslip, since these will interfere with you view of the specimen.
4. In figure 2 draw a picture of the letter "d" seen on the slide.
5. Clip the slide into place on the stage of the microscope and position it so that the letter "d" is directly over the center of the stage opening.
6. Look at the microscope from the side and use the coarse adjustment knob to lower the body tube until the low- power objective lens almost touches the slide.
7. Looking through the eyepiece use the coarse adjustment knob to raise the body tube until the specimen comes into view.
8. Turning no more than ¼ of a turn, use the fine adjustment knob to focus the letter clearly.
9. In plate 1, draw a picture of the letter "d" as viewed through the microscope. Note the magnification.
10. While looking through the eyepiece, move the slide to the left. Notice which way the letter seems to move. Now move the slide to the right. Again notice which way the letter seems to moves.
11. To switch to the high- power objective lens, look at the microscope from the side. Revolve the nosepiece so that the high-power objective lens clicks into place. Using the fine adjustment knob only, bring the specimen into focus.
12. In plate 2 draw a picture of the letter "d" as seen with the high-power objective lens. Note the magnification.
13. To switch to the medium-power objective lens look from the side and revolve the nosepiece so that medium-power objective lens clicks into place. Using the fine adjustment knob only, bring the specimen into focus.
14. In plate 3 draw a picture of the letter "d" as seen with the medium-power objective lens.
Put the slide under the microscope and focus it so that you can clearly see the individual heartbeats.
One definition of a microscope is "an optical instrument that uses a lens or a combination of lenses to produce magnified images of objects." Holden Caufield can be symbolized by a microscope and its parts: the field of view, the focus, and the magnifier.
Using the scopula, take a small amount of the substance and add it to the spot plate. Add deionized water to the section with the substance. Stir to see if the substance dissolves or not. Record your observations.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
To identify the bacteria under the microscope was not easy since it was our first time identifying bacteria. After one of our trials we would pour a drop of water on to a slide, and then add Iodine Sodium over that because the Iodine helps the bacteria to show more. We would then observe the water and record our qualitative observations, which were ½ from the microscope observation and the other ½ was while watching the water...
The word microscope is often used to define an instrument used for viewing small objects, such as bacteria, minerals, and cells that can’t be seen with the naked eye by magnifying them using a series of lenses. Microscopes vary from models and styles, but each consists of similar parts including lens, eyepiece, stage, adjustment knobs, light, nosepiece, and arm. The eyepiece allows an individual to look through it to view samples. Usually the magnification of an eyepiece 10x. The arm supports the tube which connects the eyepiece to the objective lenses and the base. The objectives are referred to as the different magnification lenses that are rotated on the nosepiece. Usually one will find three objective lenses on a microscope. They consist
Then the other two lab partners set up the information needed to record the results and the chemicals that were needed at that are needed at a certain time. Also, two of the group members that had gotten the microscopes had received the daphnia magna as well. After receiving the daphnia magna in the pipette, place it on the depression slide. Note: if there is too much liquid on the slide with the daphnia, it will have more room to run around and a person will not be able to receive an adequate reading of the heart rate. So a person will need to remove some of the liquid, leaving just enough so the daphnia does not die. Once this is done place the slide on the microscope staged with a little bit of light, adjust the focus on 4X (lowest objectives) of the microscope so a person can see the heart of the daphnia. The heart is located at the posterior side of the daphnia and is a transparent structure (SLM). Once, the heart is found count the number of beats the heart makes within fifteen seconds of timing it (SLM). After, the fifteen seconds are up multiplying the number of beats (that was counted) by four, and retrieve a chemical substance which will have a letter A-K written on a bottle. When retrieving the substance turn the objectives to make a V and place two drops of the substance beside the daphnia, wait another fifteen seconds and count the heart rate again multiply this number by four
Move each knob in the proper direction to bring your scope closer to the bullseye gradually. Chances are, you will need two or three adjustments to properly zero-in your scope.
Very carefully, place the coverslip over the depression in the slide containing the Daphnia, press the coverslip down gently, until the Daphnia is restrained by the pressure of the coverslip and is stationary, also while doing this try and remove any air bubbles that form. Try to position the Daphnia in the centre of the depression. Before starting the experiment make sure the heart is visible and that you can see the heartbeat of the Daphnia.
After lens checking the technician must trace the frame. Tracing determines the needed dimensions of the finished lens. There are two methods of doing this, tracing the frame and tracing the lens. The technician determines which method will work best with a particular frame based on experience and the lab's equipment. In frame tracing the demo lenses or the patients old lenses are removed from the frame and the frame is placed face down in the tracer and held in place with specialized clips. In pattern tracing either a pattern of the needed lens shape, a demo lens, or the patients old lens is used. Using a double sided adhesive the pattern or lens is attached to a stand that will hold it in the tracer. The technician will enter relevant information into the tracer such as a job number, patient's name, or other identifying information and also specifies whether he will be tracing a frame or a pattern, and whether he is tracing the right or left side.
Place the microtome on a table with the advancement handwheel on the right hand side
First we placed the slide under the simple microscope and observed it at ten times magnification level. We each took turns looking. We then copied them as drawings into our Cornell notebooks. After that we changed the magnification to fifty times and observed the slide. We each took turns looking. We then copied what we saw into our Cornell notebooks. After that we changed the magnification level to sixty times and we each took turns looking at the slide. We then copied what we saw into our Cornell notebooks and sat down together. When we were all done drawing we spoke about what we seen and gave each other ideas on how to write our Lab Reports.
The objective lens is the largest lens, with the largest focal length out of the two, it collects light and produces a real image of a distant object. The eyepiece lens is the smaller lens, with the smallest focal length, it takes the bright light from the focus of the objective lens and magnifies the image so that it takes up a lot of space on your retina. The final image that is created by the two lenses is inverted. This is because the objective lens inverts, but the eyepiece does not. for telescopes the image needs to be the right way up, so 45 degree prisms are placed between the objective lens and the eyepiece lens, this has the effect of turning the image the correct way up. Here is a diagram of how light travels through both the eyepiece lens and the objective
Technology in the last few decades has impacted our understanding of biological entities greatly, the genome project being a prime example. The progress that biology sees follows closely with the development of new technology. It is very important to understand and visualise the composition and structures of biological materials or samples in order to extend and correlate this to the principles of life. Microscopy is a by far the most used and the most relevant technique in this regard. However the short comings in the technological aspect of this greatly limit the usage of this to comprehend the specifics.
There are a lot of things not visible to the naked eye. What most of us don't realize is that there is a world full of wonders out there; A world full of diversity and "magic." A world called microscopy. It's amazing how one single device called the microscope can let us view things that are too small for the human eye. A microscope has the power to magnify object from 100x to as much as 1000x or higher. In fact, there are many microscopes, each with their own unique capability. Examples of these are the atomic force microscope, scanning probe microscope, and electron microscope. Microscopy is the technique in which a person is able to view images of structures that are too small for the naked eye. In light microscopy, light is reflected through a series of lenses that eventually illuminate the object being seen.