The Thermus Aquaticus project’s purpose is to identify a species of thermophilic bacteria found in a local hot spring, Vulcan Hot Springs, through genetic sequencing and analysis. The polymerase gene in other Thermus bacterias has proven useful in genetic reactions. The Vulcan bacteria grows at a higher temperature than other thermophilic bacterias, giving it the potential to have a more effective polymerase gene than what is currently available. My own research has been focused on designing effective
It is used in many labs and only requires the DNA in question, primers that anneal to the beginning and end of the target genes, Thermus aquaticus, Taq DNA, a heat stable DNA polymerase and all four of the deoxyribonucleate triphosphates. There are three steps in the PCR reaction denaturation, hybridization and DNA synthesis. During these steps the DNA is separated or denatured into two
Microorganisms play an important role in our life: helps us to digest our food, decompose wastes and participate in various life cycles. They are diverse and have adapted to inhabit different environments including extreme conditions, such as hot vents under the ocean to ice caps; hence known as extremophiles. There are more microorganisms present in us than there are cells, and the various microorganisms are bacteria, viruses, fungi and protozoa. Many people associate microorganisms as death and