The increasing in the world’s population nowadays has lead to the increase in the demand for food. It is being a priority for the crops and food industries to find a solution to this matter in order to produce high amount of food and provide good quality food for the consumers. This is where the technology of plant tissue culture steps in. As Lineberger (n.d) mentions, plant tissue culture can contributes to the agriculture industry in the future and give advantageous to the growers because the mass production of plant and crops can be produced in a short period of time using the tissue collected from single parent plant. He also include that the plant can be maintained and monitored in the regulated environment like greenhouse so that the reduction in crops production cause by the seasonal change can be avoided. Plant tissue culture is referring to the “aseptic culture of cells, tissue, organs, and their components under defined physical and chemical conditions in vitro” (Thorpe, 2006, p.9). According to Odutayo, Amusa, Okutade and Ogunsanwo (2007), single pieces of plant from the stem tip, node, meristem, embryo and seed can be used for the multiplication of plant and induces in the sterile medium for its growth. As elaborated by Reed (n.d), there are three important steps involved in plant tissue culture namely preparation of explant, multiplication and transplanting (refer to Figure 1 in Appendix 1).
The first step is preparation of explant. Explant is the process to transfer the piece of tissue taken from the mother plant and place them in a tissue culture medium (Reed, n.d.). Hussey (1986) reported that “tissues such as shoot or meristem-tips normally have an adequate covering of leaves or scales to prot...
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Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
Arabidopsis thaliana (L.) Columbia ecotype suspension- cultured T87 cells were maintained at 22°C in JPL3 medium with continuous illumination and shaking at 100g. Two-week-old cells were sieved through 500 μm stainless mesh and the remaining filtrate was transferred to a flask containing 20 ml of fresh JPL3 medium for subculture.
The focus of this lab on planaria regeneration and development. Having been taught the gradient of morphogen and there were many experiments testing this possible phenomenon of regrowth. In a planarian there is a single adult stem cell type called the neoblast. Neoblast are abundantly present throughout the body and it divides continuously. This neoblast has the ability to regenerate different cell and organ types in the planarian, from the brain, digestive system, the sensory system to even the reproductive system. With this continuous stream of continuous division of cells it allows the cells of the planarian to be rapid in self-renewal of the entire
The project will focus on a specific region (between AGIs 18,500,000 & 19,800,000) on Chromosome V, where a gene involved in root development has been mapped.
Grafting as a means of managing disease and increasing yield has only recently begun to take off in much of the western world. In Asia, however, grafting has been an important agronomic technology since the 1920’s. (Kubota) Farmers in Asia first started grafting cucurbit species and then started grafting solanaceous plants such as eggplant and tomato. Producers in Japan and Korea readil...
Six weeks previous to the conductance of this lab, Biology 108 section,planted wheat and mustard plants according to table#1 on page 3 of the Principles of Biology 108 Lab Manual . This table depicts all of the total pots and number and type of seeds planted in the pots. It accounts for the experiments of the intraspecific competition and interspecific competition. Replicates of each pot were planted to add precision and more acceptable statistics. Therefore, there were 40 pots, that is, 20 treatments conducted twice(Ciara, 1993).
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There were many physical differences between the mutant fast plant and the wild type fast plants. As shown on the graph above, the wild type plants grew bigger and fast tean the mutants. Along with height, the wild type fast plants opened more flowers. For the experimental group, we applied the gibberellic acid on the intersection of the leaves and stems. For the controlled group, the plants had received more water to balance out the amount water given to the experimental group, which was in the acid. We specifically used gibberellic acid to show an increase in plant growth, measuring in centimeters. What surprised us most is that the GA did not have an effect on the Rosette Dwarf plants until week 3. There was an increase of growth towards the end, which raises the question as to why. Why did it take so long? How much time does it take for the gibberellic acid to kick in and affect cell division? It allows us to raise more questions and continue with our
...ince, there is a need to use for advanced novel methods of culturing plant to furnish new means for quickly propagating,conserving of endangered species and also introducing exotic plants. The production of high quality planting material of exotic nature propagated from vegetative parts through tissue culture has created new opportunities in global trading. The exotic plants are advantageous for farmers;growers; nursery owners & rural employment. As exotic plants are restricted to their natural environment; the main benefit of tissue culture technology lies on production of high quality & uniform planting material that can be multiplied on a year round basis. The plant selected for such purpose is Stevia rabuadiana Bertoni. Objectives of study:
Tissue culture allows for the growth of a plant without the use of seeds or pollination.
Take cuttings for clones before you move plants from vegetative grow area to the flowering area. Low branches are cut to increase air circulation under the green canopy. Rooted clones are moved to the vegetative growth area, and new clones are started in the cloning area using the low branch cuttings. Each cycle of growth will take from 4-8 weeks, so you can constantly be growing in 3 stages, and harvesting every 6-8 weeks.
"Home | American Society of Agronomy." Home | American Society of Agronomy. N.p., n.d. Web. 25 Mar. 2014. .