Watermelon Case Study

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Watermelon or Citrullus lanatus is an annual species containing cultivated, semi domesticates and wild forms, widely distributed in tropical and subtropical areas (Jeffrey, 2001). It is consumed frequently as a dessert, fruit salad and used in garnishing drinks and produce a natural source of antioxidants (Alim-un-Nisa et al., 2012).
For the watermelon juice, the storage time used is 0 hours, 4 hours, 8 hours and 12 hours. For the 0 and 4 hours storage time of watermelon juice, the dilution for plating is 10-0, 10-2, and 10-3. All of the dilution plates is duplicate to get more accurate result. The colony counting will be carried out after 24-48 hours.
For watermelon juice with 0 hours storage time for both of the sample, it shows that many …show more content…

It also serves as a good medium for the growth of many microorganisms and bacteria. Bacterial contamination of raw milk can originate from different sources, including low quality raw milk, improper refrigeration before handling and an inadequate packaging system and the type of packaging the have been used to pack the milk (Richter et al., 1992; Torkar and Teger, 2008). Raw milk deteriorates in few days and pasteurized milk with high temperature short time system (HTST) has a shelf life of about seven days from it first open (Meunier-Goddik and Sandra, …show more content…

The CFU/ml obtained for the dilution is >3 x 102 (Est.). For the second dilution that is 10-1, the viable cell form on the first plate is 190 colonies. The CFU/ml for this plate is 1.9 x 103 CFU/ml. for the second plate with same dilution, the viable cell form is 72 colonies. The CFU/ml for this second plate is 7.2 x 102. The range that the CFU/ml should be is 0-9×104 CFU/ml and the CFU/ml obtained is in the range (Salman and Hagar, 2013). For the third dilution, both of the dilution shows no growth of viable cell. The CFU/ml for the dilution is <1.0 x 102 CFU/ml. Milk spoilage is an indefinite term and difficult to measure with accuracy (Lu, 2013). Consumers currently determine milk spoilage by checking the “sell by” and expired dates on milk cartons provided by suppliers. These dates are simple estimates of milk shelf life and their quality and are often inaccurate due to the variable processing, shipping, environment, temperature and storage conditions of the milk itself (Kim, 1997). Coliforms can cause rapid spoilage in milk because they ferment lactose with the production of acid and gas, and they can also degrade milk

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