The Effects of Sucrose Molarity on Cells in the Stem Tuber of a Potato
Planning
In this investigation I am trying to find out what molarity of sucrose
solution is same as the molarity of sucrose in the cells of a potato.
In this experiment I am going to change the molarity of the sucrose
solution out the cells of potato.
I predict that if the molarity of sucrose solution outside the cells
is isotonic to the molarity of the sucrose inside the cells then there
will be no change in weight of the potato disks. I predict this
because if the molarities are isotonic inside and outside the cells
then there will be a balanced osmotic movement of the water molecules
from inside and outside hence there will be no change in weight. I
also predict that if the molarity of the sucrose solution is
hypertonic outside the cell than the inside then there will be loss in
weight because osmosis is the movement of water molecules from an area
of high concentration of water molecules to an area low concentration
of water molecules and if there is there is a higher concentration of
sucrose solution outside then the water molecules will diffuse outside
the cell, which suggests that there will be loss in weight. Vice versa
if there is hypotonic concentration of sucrose solution outside the
cells compared to inside then there will be gain in weight.
Sucrose Molecules
[IMAGE]
[IMAGE]
Text Box: Water Molecules [IMAGE]
Below is the method that I am going to follow through my preliminary
investigation.
1. I will take a potato and cut it into half
2. Using a hole borer I will then take one cylinder of potato tissue
from the potato.
3. I will then cut the cylinder into four disks and I will then
weigh them using an electronic balance. In order to get accurate
weights I will weigh the filter paper and the cell sap on it with
then cut up 5 pieces of potato and weigh them to make sure they all
Cut a 5 cm length of celery stem 2. Cut through the grooves to divide the stem into thin strips 3. Dry the cell sap from the strips using a paper towel 4. Record and note the mass of each strip 5. Collect 6 test tubes, and put 10 cm³ of solutions 0.0, 0.1, 0.2, 0.3, 0.5, 0.7, 0.8.
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
cork borer and a ruler. I will keep the potato chips the same size in
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
there would be no flow of water into or out of the cell so the cell
repeat the process 3 times in total to ensure a fair test. At the end
I am going to use a range of concentrations to enable me to get a good
Influence of Temperature on the Activity of Potato Catalase Hypothesis That the higher the temperature the higher the reaction rate of potato catalyse to a point were denaturing occurs in the enzyme and the reaction rate of the potato catalase drops off. Prediction The rate of Catalase activity will be faster at higher temperatures until a point, because at higher temperatures there are more chances of collisions between the enzyme's (Catalase) active site and the substrate (hydrogen peroxide). However the rate depends on the active site being able to join with the substrate, and at higher temperatures the enzyme can be denatured, which changes the shape of the active site which thus prevents the reaction from happening. At first, as the temperature increases the activity of the Potato catalase also increases this is because the collision rate of the enzyme with the hydrogen peroxide is increased.
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells