The Effect on the Rate of Respiration of Yeast Cells with Glucose when the Temperature is Varied

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The Effect on the Rate of Respiration of Yeast Cells with Glucose when the Temperature is Varied

Aim

The aim of the experiment is to investigate the effect of temperature

on the rate of respiration of yeast cells with glucose. As yeast cells

use up glucose in respiration, carbon dioxide gas is given off.

Measurements of the volume of carbon dioxide gas given off within a

set amount of time can be used to measure the rate of reaction. A fast

rate of reaction would be indicated by a large volume of carbon

dioxide gas being collected within this set amount of time. A small

volume of carbon dioxide gas collected within the same amount of time

would indicate a slower rate of reaction. The rate of reaction will be

fastest at the optimum temperature for working enzymes in yeast cells.

Procedure for preliminary experiment

List of apparatus

A packet of fast acting yeast

A tub of D-glucose

Distilled water

A spatula

A rapid weighing balance

A 100ml measuring cylinder

A conical flask

A count down timer

A gas syringe

A delivery tube with rubber bungs tightly fitted at the ends joined to

the gas syringe

2 clamp stands

An electrical water bath

Method

The equipment above was collected and set up as shown below and the

electrical water bath was set to 20ºC:

· 25g of yeast and 12.5g of glucose were measured out into a 100ml

beaker using a weighing balance.

· Using a measuring cylinder, 50ml of distilled water at room

temperature was measured.

· Pour this into the beaker of yeast and glucose.

· Dissolve the yeast, glucose and water together by stirring with a

...

... middle of paper ...

...ate of reaction will be slow giving me inaccurate results. I will

control this by trying to complete my experiment in one lesson so I

can use the same yeast each time).

We had to keep the beaker rapped in tin foil so that the temperature

would stay as close as possible to the temperature that we were

measuring. The tin foil acts as an insulator and keeps the heat inside

the water.

the same thermometer during the whole experiment the results would be

inaccurate, as I would have to wait for the thermometer to reach the

certain temperatures again.

I made sure that I used the same amounts of yeast accurately, so that

I could get the most accurate results. Upon looking back at the

adjustments made, I believe that I am confident in saying that, I made

sure that my experiment was as fair as I could possibly make it.

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