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Effect of temperature on beetroot membrane
Ap bio the structure of membranes
Effect of temperature on beetroot membrane
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The Effect of Temperature on the Permeability of Beetroot Membrane
Analysis
The graph shows the colorimeter readings increase as the temperature
increases, they increase by the most at higher temperatures. This is
shown by a smooth curve.
This means that the beetroot samples release more dye at higher
temperatures. This is because higher temperatures cause the membrane
structure to break down.
The membrane structure:
Membranes have two layers of molecules called phospolipids to make up
their structure. Phospholipis consist of a glycerol molecule plus two
molecules of fatty and a phosphate group, this looks like a head with
two legs, their head is attracted to water, this means theyÂ’re
hydrophilic. The rest of the lipid (the fatty acid legs) is
hydrophobic, which means they repel water. In aqueous solutions the
phospolipids automatically arrange themselves into a double layer so
that the hydrophobic legs are packed inside the membrane (away from
the water) and the hydrophilic heads face outwards into the aqueous
solutions. The molecules are represented in the ‘fluid mosaic model’
shown below (the red bits are the legs of the phospolipids):
[draw diagram here]
If it was only made from phospolipids the membrane would be a barrier
to water, this is why there are other components scattered throughout
the phosphlipid by-layer.
Glycolipids are lipids which have combined with polysaccharides. These
may be involved with cell recognition but their exact role is not yet
known. They are found in the outer layer of cell membranes.
Glycoprotiens (seen in green on the diagram) are proteins with
polysaccharides atta...
... middle of paper ...
...root until the excess dye is completely
washed off (at least for three minutes)
- Everyone should vary the place in the beetroot they extract the
cylinders from to be cut
- The classes cylinders should all be cut by the teacher (or any other
person) to ensure there is minimal variation between the thickness of
the discs.
Conclusion
The experiment was successful as it clearly showed the effect of
temperature on the beetroot membrane. It showed that the membrane
becomes more permeable at higher temperatures, as was expected. This
was explained by the fact that proteins denature with high
temperatures and the phospholipids structure changes and becomes less
stable, having devastating results on the membrane structure. Despite
the limitations and errors, the results of the experiment were
conclusive.
2. A test tube was then filled with 35ml of yeast and placed in the
is put in pure water it will become turgid and it will not burst due
Investigating the Effects of Varying Sugar Concentration on the Amount of Osmotic Activity Between the Solution and Potato Tubes
Above in table 4.1 the results are shown. Test tube 2 was the only tube that had a change in color. The reason that test tube 2 was the only one that changed in color was because a reaction that was produced by catechol oxidase. Tube 2 was the only tube that had potato extract and catechol this is why the reaction occurred. The potato extract and catechol was not present at the same time in the other tubes and that is the reason they had no change.
potato. To make it a fair test I will make sure that the tests will be
Chlorophyll B was the least soluble because of its polarity, so it was not that soluble in hexane and ethyl acetate. Chlorophyll b was also the most polar because it contained the most oxygens within it. The oxygens make the pigment polar because of oxygen’s electronegativity. The second most polar pigment was Chlorophyll a, it was the 3rd most soluble. Chlorophyll a has five oxygens. Xanthophyll contains a single oxygen and was the second most soluble. Beta Carotene was the final pigment and it was the least polar and most soluble because it did not have any oxygens in it. There was a trend in the movement of the color bands, representing the characteristic of the polarity of the pigment. Using a nonpolar solvent, the nonpolar color bands move
Pipet chill 0.5M sucrose onto the chloroplast pellet. Use a wooden stick to mix the pellet with the sucrose until it is completely resuspended. Pipet the resuspended pellet into microcentrifuge tube labeled “P2. and keep the tube on ice. For the next steps, pipet DCPIP mix into cuvettes 1- 6. And pipet deionized water into cuvette Pre-warm the cuvettes for 5 minutes in a 37C water bath and set the spectrophotometer to 620 nm. Measure the initial absorbance of cuvettes 1-6 using cuvette 7 as the blank. Place cuvettes 2, 4, and 6 in a dark, 37C incubator. Place cuvettes 1, 3 and 5 in a test tube rack with a 100W light bulb. Read the absorbance of cuvettes 3 and 5 EVERY MINUTE for the next 10. Measure the absorbance of the dark incubated cuvettes 2, 4 and 6 after 15 minutes. After conducting the isolation of chloroplast and the photosynthesis assay of both plant sources, out of red cabbage and spinach, the spinach had a constant decrease of absorbance compared to red cabbage were it varied as the minutes passed by. Also as a result, for the red cabbage there was not a noticeable pellet like their was in the
The chlorophylls showed to have a relatively low Rf value with a range of 0.23-0.5 for chlorophyll a and chlorophyll b. The reason for the chlorophyll’s lesser mobility on the column chromatography and their lower Rf values lies in their structure. The chlorophyll consists of polar components in majority and interacts with the polar alumina in the chamber and is therefore slower to run down the chamber. With the thin layer chromatography, a similar incident occurs as the polar chlorophyll interacts with the polar absorbant in the TLC paper and the polar solvent and therefore it does not climb the TLC paper as fast as a nonpolar solvent would. The carotenes have the opposite occur and travel faster along the column and TLC paper, and therefore have a higher Rf value due to their nonpolar quality. Spots 1A and 1B on the TLC paper were hypothesized to be carotenes due to their high Rf value (0.87) and their yellow-orange color. Spots 3,5, and 12 were hypothesized to be Chlorophyll pigments because of their lower Rf values and the green/ blue-green hue of the spots. Spots 2A and 2B were hypothesized to be Pheophytin because of their distinct gray hue, although further analysis is necessary to determine if they are Pheophytin a or b. The rest of the spots were hypothesized to be various xanthophylls due to their high quantity of spots and the yellow color of the spots. Spot 4 was not distinct enough to propose an identity
The prices of the plants is $1.00 per flower or vegetable plant and $1.25 for a heirloom plant. These numbers are based off a survey Rita created, asking a selected few how much they would pay for these plants. Based off this information, and the cost of supplies, Rita was able to determined a price. This price is lower than local or all natural competition, but it is not lower than large department or hardware stores. Despite this, Rita feels that price is not a huge factor in this industry. Naturally, a customer would rather pay extra for a plant that was healthy and large vs. a small, dry, and wilted plant. She believes that because of this, she still has an advantage over the competition.
there will be in the potato chip and it will weigh less , I predict
The effect of temperature on the beetroot membranes Aim of the research: The aim of this investigation is to determine what kind of effect will the increasing temperature have on the plasma membrane of a beetroot cell. Introduction The beetroot contains a red pigment that is kept in the cells by the membranes. If the membranes are damaged, the pigment “betalain” will leek out. The amount of pigment that leeks out can be assessed, as “betalain” will colour any water that surrounds the cell.
The data in both Table 2 and Graph 1 show that as the concentration of sodium chloride (%) is increased from 0% to 10% when there is a significant increase in the % change in mass, thus also the rate of osmosis. Between concentrations 0% and 2% there was a rapid % change in mass. At 0% NaCl the % change in mass was +1.63% indicating that the potato piece had gained water because the concentration of solute must have been higher in the potato than in the solution. The % change in mass at 2% NaCl concentration was -8.82%, the negative indicates that the potato piece had lost water as the concentration of solute was higher in the solution than the potato piece. From 2% to 4% NaCl solution the %change in mass was slightly less rapid but still had an increase from -8.82 to -14.7, respectively. The graph had a slower decreasing trend from 4% NaCl solution onwards, this can be seen from the gradual plateau of the graph. The slowest increase in %change in mass of -0.04% was between 6% to 8% valued at -16.5% and -16.9%, respectively. The % change in mass increased slightly from -16.9% to -17.5% between 8% to 10% NaCl concentration. This suggests that the rate of osmosis had begun to stabilize and may be the result of the concentrations of both the
Investigating Osmosis In A Potato Introduction: "Osmosis is typically defines as the flow of one constituent of a solution through a membrane while the other constituents are blocked and unable to pass through the membrane. Experimentation is necessary to determine which membranes permit selective flow, or osmosis, because not all membranes act in this way. Many membranes allow all or none of the constituents of a solution to pass through; only a few allow a selective flow. In a classic demonstration of osmosis, a vertical tube containing a solution of sugar, with its lower end closed off by a semi-permeable membrane, is placed in a container of water. As the water passes through the membrane into the tube, the level of sugar solution in the tube visibly rises.
with 0.0M (distilled water) and go up by 0.1M until I reach 1.0M and I
· The beetroot piece is then placed into a tube of 5 cm of distilled