The Effect of Temperature on the Hatching Success of Brine Shrimps
In this experiment I will study brine shrimps and test the hatching
success of them in different temperatures. These crustaceans are small
salt water ones; so small that the adults are only about 8 mm in
length. They are relatively easy to keep in the laboratory and will
produce dormant egg cysts that hatch to produce young shrimp larvae.
Hypothesis
It is very hard to come to a prediction about this type of experiment
as we do not know enough about them to see what affects the
reproductive ability of them.
Safety
1. Take care with the glassware make sure that they do not break.
2. The magnifying glasses as they are fragile also.
Apparatus
• 100 cm3 beakers (one for each temperature to be tested)
• 100 cm3 de-chlorinated water for each beaker
• 2 g sea salt for each beaker
• Stirring rod
• Access to refrigerator
• Water baths or incubators (one for each temperature to be
investigated)
• Brine shrimp egg cysts
• Sheet of graph paper 3 cm _ 4 cm
• Magnifying glass
• Pair of forceps
• Bright light
• Fine glass pipette
• 40 cm3 beaker of salt water
Procedure
1. Decide on a range of temperatures from 5 °C to 35 °C to be tested.
2. Place 2 g of sea salt into a 100 cm3 beaker.
3. Add 100 cm3 of de-chlorinated water and stir until the salt
completely dissolves.
4. Label the beaker with the temperature at which it will be
incubated.
5. Place a tiny pinch of egg cysts onto a large sheet of white paper.
6. Wet the square of graph paper using a few drops of salt water. Dab
the paper onto the white sheet to pick up about 100 eggs. This will
look like a tiny shake of pepper. Use a magnifying glass to count the
2. Drop a gummy bear into each of your prepared beaker or cup and place the beaker or cup
Firstly, when testing temperatures at 30°C and 40°C, the water was. sometimes heated more than needed, so I had to wait until it cooled. down to the required temperature. To avoid this happening, a. thermostatic water bath could have been used, because I could set it. to the required temperature.
The temperatures will be set up by using ice to cool tap water down to lower temperatures and boiling water to heat it up. The temperature will be measured to within 0.1°C on a mercury thermometer to ensure accuracy. The temperatures will range from 10°C to approximately 40 °C and it will be done at 5° C intervals.
In the early development process of many organisms, it is important to be able to minimize exposure to agents of stunted or arrested development. By decreasing the mortality rate for a generation of a species, that species is given an advantage in later reproduction; by increasing the number of organisms of the same species within a limited environment, more organisms of the same species are able to reproduce, resulting in an augmented overall population ("Reproduction and Development", 2013). However, when toxins are introduced to an environment, an embryo’s viability can decrease. Mortality rates for the generation of the species can increase, and defects that are harmful to the reproductive cycle can emerge. Thus, it is necessary to measure and observe the effects of certain toxins on embryonic development. The North American brine shrimp, or Artemia Franciscana (Artemia Salina), is subject to changes in its environment. Toxins introduced to its hatching environment, such as ethanol (in concentrations of 0.1%, 0.15%, and 0.2%), can have significant impact for the hatching process and embryonic development. The experiment sought to explore the relationship between birth defects and exposure to ethanol at early developmental stages through the use of American brine shrimp. However, to be able to fully comprehend the impact that certain toxins would have on the embryonic development of the North American brine shrimp, it is first important to be versed in its specific hatching process.
I will make it a fair test by having all variables such as heat and
We must first begin the today’s lab by connecting the thermometer that digitally detects surrounding temperature to the Lab Pro Interface located on the computer via...
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
Plan 1. Collect 4 different sized beakers 2. Boil some water in the kettle 3. Pour 50ml into each beaker 4. After 1 minute check temperature 5.
5.) One at a time, place your test tubes in the water bath and heat the first test tube to 25 , the second to 50 , the third to 75, and the last to 100 degrees c. Remeber to stir with your stirring rod every so often.
Shrimp can be very intelligent animals and have very amazing things such as being clear, this may seem weird but yes, they are clear. Shrimp live in the ocean and come in contact with many different plants animals. The most common plant that the shrimp comes in contact the most with and use for protection is seaweed, coral, and algae. Shrimp use the for protection to hide from the other animals they depend on shrimp for their diet, most of these animals are sharks, whales and sometimes crabs are included. The change of having no eyes helps the shrimp hide from these and keeps the population at a good rate and this has also helped overfishing with not having contact with other animals and causing lots of movement to the other animals and fisherman.
- Temperature was measured after and exact time i.e. 1 minute, 2 minutes, 3 minutes.
An art so ancient yet so new to the human race is the art of the kitchen. All nations without any exception, old or new, large or small, have given and give their valuable help for the further expansion of the art of the kitchen, or as we are used to call it, the art of cooking. One of the most important factors that influences as a safeguard for a person's health and his abilities to work, is food. Food is the basis of life, an energy source without which life would not be possible. It is indispensable that a person's food should contain all of those nutrients that are needed for our organism, such as: proteins, fat, carbohydrates, vitamins, mineral salts and ferments.
Osmoregulation is an example of an organism maintaining homeostasis. More specifically, osmoregulation involves an animal regulating osmotic pressure, or its fluid content. Brine shrimp, Artemia, use osmoregulation to regulate the saline levels of fluid within their body. Because brine shrimps live in seawater, an environment with a high saline concentration, they must actively excrete excess salt. Brine Shrimps use metepipodites as the location of the ion pump which secretes sodium. This is an active transport of ions because it is moving against the gradient, a higher salt content outside the body. The two following studies describe the environmental conditions ideal for brine shrimp and the possible genetic explanation for the osmoregulation of brine shrimp, respectively.
Methodology: A plastic cup was filled half way with crushed ice and mixed with four spoonfuls of 5 mL of sodium chloride. A thermometer was quickly placed inside the cup to take the temperature and the