The Effect of Temperature on the Action of Protease on Photographic Film Aim: to show the effect of temperature on the action of protease on photographic film Prediction: I predict that as the temperature of the enzyme increases, so will the rate of reaction. However, I only predict this until a certain temperature and beyond perhaps 60(optimum temperature) the enzyme will stop working as well and both the enzyme and the substrate will become slightly deformed. As the temperature rises, molecules move much faster and as a result have more energy. This means that they will be more likely to collide so overall increasing the temperature will increase the chances of successful collisions. However with enzymes at the temperature of perhaps 70-75 degrees, their protein structure will break down and their shape may become slightly deformed. This means that they won't be able to fit into the substrate anymore, slowing down the reaction and eventually stopping it. Preliminary experiment In order to familiarise myself with the experiment, I did some preliminary work. I carried out the exact same experiment that I am hoping to do for my final experiment. I measured up 10cm3 of protease enzyme using a syringe (an accurate form of measurement) and placed it in a test tube. I then placed this in a water bath of the required temperature for 3 minutes before placing in the photographic film, to bring it up to the necessary temperature. The stop watch was switched on and the photographic film was then placed in the test tube, attached to a splint and was taken every now and again to check if the film had turned transparent. Once... ... middle of paper ... ...fect of temperature of the action of a different protease, say pepsin on a photographic film. This is because; I could then compare the effect of a bacterial enzyme against a human one. I could also use a wider range of temperatures, to add detail and to be able to draw a more accurate graph based on the results. In addition I could investigate more temperature around the optimum temperature, to find the exact temperature at which the enzyme is most efficient. In order to make the results more reliable I could also do more repeats, or do repeats in separate test tubes, unlike the experiment that I carried out. Both repeats were in the same test tube, which means they could be classed as not repeats, however If I were to carry out repeats in two different test tubes, I would get similar results anyway.
5. A second test tube was then filled with water and placed in a test
The procedure of the lab on day one was to get a ring stand and clamp, then put the substance in the test tube. Then put the test tube in the clamp and then get a Bunsen burner. After that put the Bunsen burner underneath the test tube to heat it. The procedure of the lab for day two was almost exactly the same, except the substances that were used were different. The
The Effect of Temperature on an Enzyme's Ability to Break Down Fat Aim: To investigate the effect of temperature on an enzyme’s (lipase) ability to break down fat. Hypothesis: The graph below shows the rate increasing as the enzymes get closer to their optimum temperature (around 35 degrees Celsius) from room temperature. The enzyme particles are moving quicker because the temperature increases so more collisions and reactions occur between the enzymes and the substrate molecules. After this the graph shows the rate decreasing as the enzymes are past their optimum temperature (higher than). They are getting exposed to temperatures that are too hot and so the proteins are being destroyed.
Experimental: The experimental procedure outlined in the OU Physical Chemistry Laboratory Manual was followed without any deviations.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
This reduces recovery time and may increase the rate of success due to fewer traumas to the connective tissue. And also, it has a small scar.
The film Declining by Degrees effectively argues its claim that all is not right in higher education. They do this by interviewing countless professors and students that still attend college or that have recently graduated or dropped out. Their use of personal experiences, statistics, and expert opinions helps build their credibility and emotional appeal for the viewers of the documentary. The main audience for this documentary being anyone who cares about college, parents, students, and even the professors and staff at colleges in the United States.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
...e foot, asks the athlete exactly what happened and is informed that there was a ‘snapping’ noise heard at the time of injury. Due to swelling it would be difficult to palpate the joint line if the ankle, but there is pain on palpation. The therapist will direct the athlete to perform active movements of the ankle; the movements were not produced by the athlete due to the amount of pain. The next step is to get the athlete off of the pitch safely. Due to the lack of active movement there will be hesitation to apply full pressure on the ankle and the unusual positioning it would be best for the therapist to splint the ankle with a SAM splint and then remove from the pitch according to the EAP(see appendix 1&4) (Wilkerson, A.J. etal (2010).
The first step was to add 1 mL of a premade 8% sodium chloride (salt) solution to a large test tube. One mL is approximately 20 drops from a pipette. The salt solution is very important because it will go into the DNA and destroy the histones that the DNA is wrapped around (Rice, George). It will also start to break apart the cell membrane so we can get to the DNA (DNA Extraction Techniques).
In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.
Wear the splint as told by your health care provider. Remove it only as told by your health care provider.
2. In the large beaker, put water and boil it completely. After that, remove the beaker from heat. 3. Sample tubes (A-D) should be labeled and capped tightly.
Pegs that fastened my left Arm to the Ground; for, by lifting it up to my Face, I
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and