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Recommended: Catalase activity
At the time of conducting the experiment, the intention was to analyse the effect that an increase in catalase concentration had on the amount of oxygen (O2) produced if the hydrogen peroxide (H2O2) concentration was constant. It was proposed that when the catalase increases in concentration at the increments of 0.5%, 1%, 2%, and 4% and the hydrogen peroxide remained at a fixed concentration, the oxygen production would rise gradually. It was suggested that this would occur due to the collision theory ("BBC - GCSE Bitesize: Collision Theory") and the greater number of active sites thus an increase in accessibility allowing enzyme- substrate complex’s to be formed. According to the data gathered from the experiment, it can be implied that the hypothesis was supported as the data indicates an incline in oxygen …show more content…
This is evident as the average gradient for 0.5% catalase concentration is 0.067%/s and the average gradient of 4% catalase concentration is 0.315%/s. The rise of oxygen production has occurred because as the hydrogen peroxide is added to the varying levels of concentration of catalase. The solution with the highest enzyme concentration will yield the most activity, thus producing more oxygen gas ("Enzyme Investigation. How Does The Concentration Of Hydrogen Peroxide Affect The Amount Of Oxygen Produced By The Enzyme Catalase? - International Baccalaureate Biology - Marked By Teachers.Com"). This assumption is based on scientific theory, which states an increase in enzyme concentration will also increase the chances of substrate-enzyme binding.("General Principles - Chemwiki") An example of this is the average R2-value for 1% catalase solution was 0.090%/s and the average R2- value of the 2% catalase solution was 0.094%/s. This indicates a strong positive correlation, meaning that as x increases (catalase concentration), y also increases (rate of oxygen
The purpose of this study is to analyze the activity of the enzyme, catalase, through our understanding
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Hydrogen Peroxide does not need the enzymes in the catalase to separate into Oxygen and Water. The catalase are only used to increase the rate of reaction.
Because of its undeniable and scientifically-proven powerful antioxidant properties, catalase enzyme has a wide commercial application. Primarily in combination with the enzyme glucose oxidase, catalase is used as a preserving system in mayonnaise and egg products such as whole eggs or dried egg white, in the preparation of milk and cheese, in the manufacture of baked goods, beverages, textile industry, cosmetic industry (as face mask), and in cleaning agents for contact lenses, for the elimination of the hydrogen peroxide that is present in some products, (Worthington Biochemical Corporation,
How does the temperature (-2°C, 20°C, 30°C, 40°C, 60°C) affect the production of oxygen (cm3) from cow hepatic (the enzyme catalase) when placed in boiling tube with 10 ml of 3% hydrogen peroxide for 1 minute?
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
Enzymes are substances (most often proteins) that act as biological catalyst. A catalyst speeds up the rate of chemical reaction. This process promotes the conversion of reaction into products. Most of us know that hydrogen peroxide is a toxic chemical but catalase speeds up the breakdown of hydrogen peroxide into non-toxic substance such as water and oxygen. In this catalase enzyme vs. hydrogen peroxide experiment we ask, what environmental treatment will reduce catalase enzyme activity in a potato? We will discover how a potatoes temperature including hot, cold, and room temperatures will affect the catalase enzyme activity. With this information in mind we hypothesized the potato that
To investigate the amount of oxygen foam (cm) produced by the enzyme catalase when it breaks down hydrogen peroxide in the animal (liver, milk, honey) and plant cells(potato, purple cabbage) into oxygen and water
Aim: in this experiment is to use this reaction to see what substances have high levels of the Catalase enzymes and if different acids and basses are added would affect the reaction and the enzyme.
The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase. A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Introduction This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects that you submit for marking.
Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with different concentrations of the substrate. Upon reviewing observations, it can be concluded that liver contains the greater amount of catalase as its rates of reaction were greater than that of the potato.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells Introduction Enzymes are made of proteins and they speed up reactions, this means that they act as catalysts. Hydrogen peroxide is a byproduct of our cell's activities and is very toxic. The enzymes in our bodies break down the hydrogen peroxide at certain temperatures they work best at body temperature, which is approximately 37 degrees. At high temperatures, the cells begin to denature. This means that the hydrogen peroxide is prevented from being broken down because they will not 'fit' into the enzyme.[IMAGE] Objective I am going to find out how the concentration of the substrate, hydrogen peroxide affects the reaction in the catalase inside the potato cells.
The enzyme used in this lab is catalase. Catalase has a molecular weight of approximately 240,000 daltons and contains four polypeptide chains, each composed of more than 500 amino acids. This enzyme occurs universally in aerobic organisms. One function of catalase within cells is to prevent the accu...
Without enzymes, reactions wouldn’t occur and living organisms would die. For instance, the enzyme in the stomach breaks down large molecules to smaller molecules to absorb nutrition faster. Researchers experimented with enzyme activity with a potato extract. Researchers will test enzyme activity by increasing and decreasing pH levels, lowering and increasing temperature, and substrate concentration effects. In the first experiment, researchers hypothesized whether different pH levels would change how much Benzoquinone are created and how will the enzymes function in neutral pH levels than higher and lower levels. Researchers used potato extract and different levels of pH to test their hypothesis. In addition, researchers questioned at what temperature does the greatest amount of potato extract enzyme activity take place in. Researchers then hypothesized that the results would indicate the greatest amount of potato enzyme activity level will take place in room temperature. In this experiment, researchers used potato extract and different temperature levels to test the hypothesis. Moreover, researchers wanted to test the color intensity scale and how specific catechol oxidase is for catechol. In this experiment, researchers used dH2O, catechol solution, hydroquinone, and potato extract. Lastly, researchers tested the substrate concentration and how it has an effect on enzyme activity. In this experiment researchers used different measurements of catechol and 1cm of potato extract. Researchers hypothesized that the increase o substrate would level out the enzyme activity