Testing and Evaluating the Contents of Two Known Solutions for Proteins and Lipids
Introduction
For this experiment two solutions will be provided. In one test tube
it contains milk and in the other test tube it contains sunflower oil.
The test for proteins and lipids will be done for each solution and
then a conclusion can be deduced from these results.
To test for the proteins place 2cm³ of the test solution into a test
tube and then add five drops of the Biuret solution to it. It is
important to known what it is your looking for , in this case to show
that there are proteins present it should develop a purple- violet
colour, the intensity of which is proportional to the content of
protein, with a high content of protein it will give a darker
precipitate rather than if it had a low content where the precipitate
will be lighter in colour. However if in doubt about the colour add
more drops to it , wait a few moments a purple colour will develop if
a protein is present. The reason why there is this colour change is
because in because of the nitrogen atoms in the Biuret solution
present in the peptide chain form a purple complex ( the nitrogen)
with the Cu ²+ ions, it is the dilute copper sulphate that is in an
alkaline solution.
For the test for lipids I can use two tests. The first test being the
translucence test where a drop of each solution is put on a piece of
filter paper, if lipids is present than it should turn the filter
paper translucent this because of the oils in the triglyceride chains
causes this translucent effect on the filter paper.
However if this test proves unsuccessful and not clear another test
c...
... middle of paper ...
...oduce a green then
to orange precipitate. The reason why this is because when the
benedict's test was done for the non reducing sugar the lactose it at
first had no effect, then the solutions would be neutralised and then
dilute hydrochloric acid would be added to them. When lactose is
heated with acid you hydrolyse it into the monosaccharide that it is
made up of ( for lactose this is galactose and alpha glucose). So then
when it is heated with the benedict's solution it gives you a reaction
because now it can work as there are reducing sugars present that
allow it to give red precipitate. The reason why test it would now go
orange is because it contains non reducing sugars so when the non
reducing sugar are heated it hydrolysed into twice as many
monosaccharide- reducing sugars than there were of non reducing sugars.
In this case, the nitrate ion moved from one compound to another as the Cu was replaced by Zn. The zinc dissolved to form zinc (II) ions as the copper (II) ions came out of the solution as copper metal and deposited on the surface of the
The purpose of this lab is to determine the empirical formula of copper oxide (CuxOy) through a single-displacement reaction that extracts the copper (Cu) from the original compound. In order to do this, hydrochloric acid (HCl) was mixed in with solid CuxOy; the mixture was stirred until the CuxOy was totally dissolved in the solvent. Zinc (Zn) was then added to the solution as a way to enact a single displacement reaction in which Cu begin to form on the Zn; the Cu gets knocked off the Zn through gentle stirring. To isolate the Cu, the supernatant liquid was decanted and the Cu was then washed with first water then second, isopropyl alcohol. Once done, the hydrated Cu is transferred onto an evaporating dish where it was heated multiple times
The chemical equation associated with chemical equilibrium that we will be using in this lab is CuCl42-(aq) + 4H2O (l) ⇌ Cu(H2O)42+ + 4Cl-(aq).
...th. The test can be to check your blood glucose levels, cholesterol, triglycerides, lactic, and uric acid. And to check if your growing and checking for enlargements of the liver.
Isotopes refer to 1 of 2 or more atoms with the same atomic number but different numbers of neutrons. The atom copper has two stable isotopes. They are 63Cu and 65Cu. 63Cu has an isotope atomic mass (in amu) of 62.9295989 and a natural abundance (in atom %) of 69.17. 65Cu has an isotope atomic mass (in amu) of 64.9277929 and a natural abundance (in atom %) of 30.83. The averag...
are left in the solution, the more time there is for the copper to be
Protein is an important part of our daily diet. All human beings need it. The Institute of Medicine Recommends adults get a minimum of .8 grams of protein per kilogram of body weight. It is recommended that 10-35% of your required daily calorie intake is made up of protein. Most Americans consume more than enough protein daily, but would benefit from leaner and more varied choices in their protein selections. Protein is found in every body part and our tissues including hair, nails, skin, bones and muscle. It creates the enzymes that fuel several chemical reactions and the hemoglobin that carries oxygen throughout our blood. There are at least 10,000 proteins that make up and maintain us.
1972 fluid mosaic model. Lipids are commonly recognized as fats, oils, wax, etc. There are three
Using three test tubes with samples in instead of two test tubes with samples in.
The main building blocks of lipids are fatty acids and glycerin. The elements of lipids are the same as carbohydrates, CHO. What lipids are is stored energy. Even though carbohydrates and lipids are made up of the same elements, they are different. The difference is their structures. Some examples of lipids are fats and oils, saturated: animals, and unsaturated: plants. One of the test you could do to see if there is lipids in a food is the lipid test. This is how you do the lipid test. Step 1. Rub a small amount of sample on a square of brown paper bag. Step 2. Brush off excess food. Step 3. Let paper dry. Step 4. If lipids (fats) are present, a greasy or oily stain will show up when paper s dry and held up to the
5. Add Hcl, as soon as it mixes with the ( ), start the stopwatch
To make it a fair test I will test each concentration three times and use the average. My tests will be accurate as I will be using a very accurate scale and precise syringes. To make my experiment accurate I will be using distilled water to make my sucrose solutions so there are no impurities that may affect my experiment and I will also measure my results to two decimal places. To make my experiment safe I will use goggles.
Prepare .05 to .075 g of crude lipid by dissolving it in hexane. Add to the silica gel slurry in the column.
second test tube also add 6 mL of 0.1M HCl. Make a solution of 0.165