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Procedures and practices in a lab
General laboratory techniques 1
3 important things about good laboratory practice
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The entirety of this lab experiments was testing general basic understanding of lab procedures. It tested if I can follow the basic direction and with carefully deduction, concluded to what my unknown organism was in the end. I had no problem in the beginning and determining I was working with a Gram-positive organism. I felt my process of doing lab test grew faster over time because in the beginning, I was very slow to smear prep. However, this gradually improved over time and in addition, so did my use of the microscope. I would say I mastered the technique of my lab procedures, but more experienced and well education on the uses of the basics. In addition, my upkeep in cleanness helped my stock from getting contamination. The only trouble I encountered over the course was capsule staining and my motility test. The capsule stain is just a pain in the butt to do, and after I did this procedure twice on different occasions, there was no capsule I could find. For the motility test, my first time doing this test resulted in a …show more content…
It is also resistant to multiple antibiotics including vancomycin. Vancomycin is used as a last resort for many gram positive infections (Kau, 2005). E. faecalis is part of our body function, as commensal bacteria without disrupting normal flora (Kau, 2005). However, when a patient is administrated into the hospital a change will likely occur, causing the organism to grow. An example for this is the use of a catheter (Kau, 2005). In addition, the immune defense is suppressed while a patient is being hospitalized can attract other pathogens to ask the subject, such as S. aureus or E. coli (Draper, 2013). The rate of growth occurrence various differently based on location. As the clinical study of bacteria identification in Emergency Department in comparison to Hospital (Draper,
...imary stain and not pick up the counterstain. Other human errors could have affected the results such as not inverting the plate before putting it into incubation would not allow for bacterial growth. Not pipetting the tube up and down to mix the bacteria that settled at the bottom of the tube before starting the Gram Stain would not allow for an accurate reading because there wouldn’t be many bacteria on the slide. Passing the slide over the bunsen burner too many times, hence killing the bacteria and not allowing for a Gram Stain. If this experiment had to be redone, one improvement would be to allow for more that one plate without a point deduction. Unexpected human errors might interfere with person’s results. Having more than one plate will allow for more accuracy in the results or allow for a person to determine were they went wrong during the experiment.
I also inoculated a tryptic soy broth (TSB), a nutrient gelatin deep, a motility agar deep, a fluid thioglycollate medium (FTM) tube, and a TSA plate with my unknown culture. All of these inoculated media were incubated until the next class period (about 48 hours). Then when I came to class most of my inoculated tubes and my streak plate appeared to have growth. The next step I took was making a gram stain to determine the gram reaction and cellular morphology of my unknown. I used my working slant to do this, after careful examination of the gram stain, I learned that my unknown was a gram-positive bacterium. I then preceded by making a negative stain to see the size of the cells of my unknown bacteria. The cell shape was cocci and the cells occurred in clusters of tetrads. After discovering that my unknown bacteria was gram-positive cocci, I turned to page 207 of the lab manual to narrow down my options, there was only four out of the gram-positive list that were
I began my test to classify my unknown bacteria by performing the Gram staining because according to the first period procedure of the laboratory manual and the Appending H, it was the first test that should be done to plan and proceed to the next tests. Washed bottle of distilled water, three slides, and Gram-staining reagents
Thyroid and metabolism hormones play a large role in the daily lives of all living species. Thyroid hormones regulate the metabolism and the metabolism is responsible for maintaining a specific range for the biochemical reactions that occur in the body (Martini 2014). The most important hormone for metabolic maintenance is thyroxine (T4). This hormone also plays a large role in body heat regulation. It is produced by the pituitary gland and secreted by the thyroid gland. The thyroid releasing hormone (TRH) must trigger the thyroid stimulating hormones (TSH) to release thyroid hormones to the thyroid gland. These hormones are under control of the hypothalamus, or main neural control center. Propylthiouracil (PTU) is a medication used to treat
Aerosolized antibiotic used in this study that are proven to be effective are: amikacin, colistin, ceftazidime, gentamicin, tobramycin, sisomycin, and yancomycin. However, increasing antibiotic resistance patterns among intensive care unit pathogens, cultivated by empiric-broad spectrum antibiotic regimens, characterizes the variable concerns. Recent literature point that antibiotic use before the development of VAP is associated with increased risk for potentially resistant gran-negative infections and Methcillin-resistant Staphylococcus auereus (MRSA)
The significant increase in antibiotic resistance amongst pathogens is making it very difficult to successfully treat infections, especially in intensive care units (ICU’s). Prevention of the spread of infection among patients within the hospitals is fast becoming amongst the most important methods for controlling infections. This requires the identification of the different acquisition routes, that is, routes by which bacterial colonization occurs. In this article, the authors analyzed the relative importance of various bacterial acquisition routes that resulted in colonization of the bacteria using data from individual patients.[1] This article was chosen because of the impact it can have on the healthcare system if the knowledge obtained from the algorithm regarding the most prevalent colonization routes in hospitals can help in the preventing spread of infections. Also the ability of the algorithm to incorporate specific patient characteristics makes it both novel and appealing.
Scott, II, R. D. (2009). The direct medical costs of healthcare-associated infections in U.S. hospitals and the benefits of prevention. Retrieved from http://www.cdc.gov/hai/pdfs/hai/scott_costpaper.pdf
Scott II, D. R. (2009). The direct medical costs of healthcare-associated infections in U.S. hospitals and the benefits of prevention. Retrieved from http://www.cdc.gov/HAI/pdfs/hai/Scott_CostPaper.pdf
Rello, J., Kollef, M., Diaz, E., & Rodriguez, A. (2000).Infectious diseases in critical care. (2nd ed., pp. 352-
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the Microorganism (M.O.) #16, I prepared a slide by cleaning and drying it. Then, using a bottle of water I placed a sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes.
Health care facilities - whether hospitals, nursing homes or outpatient facilities - can be dangerous places for the acquisition of infections (EHA). The most common type of nosocomial infections are surgical wound infections, respiratory infections, genitourinary infections and gastrointestinal infection (EHA). Nosocomial infections are those that originate or occur in health care setting (Abedon). They can also be defined as those that occur within 48 hours of hospital admission, 3 days of discharge or 30 days of an operation (Inweregbu). These infections are often caused by breaches of infection control practices and procedures, unclean and non-sterile environmental surfaces, and ill employees (EHA). Immunocompromised patients, the elderly and young children are usually more susceptible to these types of infections. Nosocomial infections are transmitted through direct contact from the hospital staff, inadequately sterilized instruments, aerosol droplets from other ill patients or even the food and water provided at the hospital (EHA). The symptoms of nosocomial infections vary by type but may include inflammation, discharge, fever, abscesses, and pain and irritation at the infection site (Stubblefield).
wards. Clinical Microbiology And Infection: The Official Publication Of The European Society Of Clinical Microbiology And Infectious Diseases, 18(12), 1215-1217. doi:10.1111/j.1469-0691.2011.03735.x
Secondary:Curtis, L. (2008). Prevention of hospital-acquired infections: review of non-pharmacological interventions. Journal of Hospital Infection, 69(3), 204-219. Revised 01/20
...s and measurement to decrease healthcare- associated infections. American Journal Of Infection Control, pp. S19-S25. doi:10.1016/j.ajic.2012.02.008.
This world, or what scientist would call a “microbiome,” is one of the deadliest battle fields for workers in the hospital and healthcare fields. Thanks to the development of technology in the microbiology field, scientists are now able to view and identify the pathogens that invade the human body. Rest and fluids are not strong enough to diminish these invaders; the weapons