Micro Filtration Essay

Lit Review

Microfiltration

The process of removing particles from 0.025 micron to 10.0 micron range from fluids by passing through any microporous medium called micro filtration is, in this case, a membrane filter. In this experiment, we will be using 2 and 7-micron nanoparticles (Munir, 2006). When we look at microfiltration. We are looking at how the smallest particles can get through a filter. Specifically, backflush, which is reversed flow. Incorporating backflush into dead end filtration creates a more efficient system (Li, Bertram & Wiley, 1998).

Dead End filtration

Dead end filtration is flow applied perpendicular to the membrane surface. Particles smaller than the effective pore size pass through as filtrate, and particles that are

A rotary pump, four valves, filtration region, channels, an inlet, and two outlets make up the filtration chip aka the filter. Now, the filtration site has two fluidic chambers, divided by a microporous membrane that act as a sieve to filter out the smaller nanoparticles from the larger ones. This allows the smaller particles to go to the bottom chamber (Chen, Wang, Chen & Chin, 2010). The next most important mechanical parts are the microfluidic sample traps. Microfluidic sample traps are any device that allow to individually trap micrometer to millimeter-scale cell and tissue samples in a channel recess, hanging drop, or small chamber adjacent to a main channel where samples circulate in a carrier fluid. They are purposeful when loading samples or simply growing them within the trap from injected cell suspensions as in spheroid synthesis (Rousset, Monet & Gervais, 2017). Overall, the materials needed for this experiment are Dispensing Pressure Vessel, Stirred Cell Model 8400 200 ml, USB Connector(RS32), Microfluidic sample traps, dead end filters, syringes and a pipettor (Munir, 2006).

Polystyrene nanoparticles

Polystyrene is an aromatic polymer, made when you polymerize styrene monomers. To ensure that the nanoparticles do not degrade in the cellular environment and do appear toxic to cells, they compose the nanoparticles from polystyrene. Also, by using polystyrene particles, it is possible to

They have a larger surface area in proportion to their volume. This enables them to interact with different types of biological systems and provides a wide variety of possibilities (Nuruzatulifah, Nizam, & Ain, 2015). The nanoparticles can be seen by transmission electron microscopy (TEM). When there is one nanoparticle, it is called a primary particle. When there is more than one, it is called a secondary particle. In order to measure these particles, they need to be suspended in a solution (Pruneanu, Coros & Pogacean, 2015). Dyed nanoparticles or internally fluorescent nanoparticles barely interact with cellular proteins which is what the study requires. They are also quite easy to manipulate. They can be easily internalized into cells and can be programmed to go to specific sites (Wolbeis,