Investigating Osmosis in Potato Tissue

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Investigating Osmosis in Potato Tissue

Aim

To estimate the concentration of sucrose in potato tissue.

Hypothesis

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I believe that the potato tissue that has been in the hypotonic (0.1M

sucrose) solution will be more turgid than before, while the tissue in

the isotonic (0.3M sucrose) will be similar to its previous state. The

tissue that has been in the hypertonic (0.5M sucrose) should be far

more placid than it was before. This is because of osmosis - water

diffusing in and out of the potato tissue cells.

Apparatus

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· 1 potato tuber

· 1 cork borer

· 1 ceramic tile

· 1 scalpel

· Paper towels

· 6 boiling tubes

· 1 marker pen

· 0.1M, 0.3M, and 0.5M sucrose solution

· Access to an electric balance

· 1 funnel

Method

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The volume of each boiling tube was calculated, and then 2 tubes were

half-filled with 0.1M sucrose solution, 2 tubes were filled with 0.3M

sucrose solution, while the final 2 tubes were filled with 0.5M

sucrose solution. Then 6 cylinders of potato were cut from the tuber

using the cork borer. These were then all cut down to 20mm in length

using the scalpel. They were then dried with the paper towel to remove

excess water before being weighed on the electric balance. The weight

of each was recorded. They were then all immersed in the solution in

the boiling tubes, and the tubes were labelled using the marker pen

and then lids were put on. They were then left for 24 hours in a

moderate temperature before being taken out and dried using the paper

towels. They were then weighed on the electric balance and the final

mass was recorded. The cylinders were then gently squeezed to

determine the condition of the potato tissue after osmosis - very firm

(turgid), firm, or soft (flaccid). The percentage difference between

the mass before and after the experiment was then calculated, and the

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