Gel Electrophoresis Procedure

Procedure To begin with this project, a gel electrophoresis chamber must be built. In this chamber, a plastic box will be the chamber, a stainless steel wire will replicate electrodes, batteries will be the power outlet, and the wells will be replicated by using a styrofoam comb. Hold the plastic box horizontally.
First cut two separate pieces of the stainless steel wire with your wire cutters (Remember the gauge must be no smaller than 18 and no larger than 24!). The wire should be a few centimeters longer than the actual width of the plastic box.
Shape the wire so they have a hook that hooks over the sides of the width of the box. When you have hooked the wires, put one at the bottom being your negative electrode, and the other one at

The buffer solution will be a 1% solution of baking soda in water. To make this, combine 2 grams of baking soda with 200 mL of bottled water in a bowl and stir well.
To make a 1% agarose gel solution for the gel electrophoresis, combine 1 g of agarose with 100 mL of your buffer solution in a microwave-safe bowl.
Heat the agarose solution in a microwave to dissolve the powder. Stop the microwave every 10-15 seconds to stir the solution.
After you see that the solution is starting to bubble, remove it from the microwave. At this point the solution is now translucent. Remember to watch the agarose solution closely and remove it carefully. When it gets hot, bubbles will form and you will be able to remove it.
Since we know need to insert the comb in the liquid gel, remove the stainless steel wire electrodes form the chamber.
At this point, insert your styrofoam comb into either end of the gel, while leaving approximately 5 millimeters between the comb and the end of the box. Pour the agarose solution into the gel chamber, till the comb teeth are submerged by 10 millimeters. If the gel is too thick, the project may not have any results that can be