Factors that Affect the Rate of Respiration in Yeast

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Factors that Affect the Rate of Respiration in Yeast Introduction ============ Yeast is one of the living cells, which can respire aerobically, and anaerobically in this investigation I am just taking into consideration respiration as whole. The equation of respiration in yeast is: Equation: There are different variables in this experiment. These consist of the range in temperature, concentration of glucose, amount of hydrogen carbonate solution used and amount of water used for the water bath. These variables are important and affect the way in which yeast respires. I shall be choosing the range in temperature as the variable to study to see if it affects the rate of respiration. Prediction ========== In this experiment the temperature shall affect the way in which yeast respires. I predict that the temperature at which the rate of reaction shall be fastest is at higher temperatures but below 50ºC. This is because enzymes work best at the higher temperatures. If the temperature is above around 50ºC enzymes break down and stop working causing for respiration not to take place. However if the temperature is less than around 30ºC it shall mean that the enzymes work slower and therefore the reactions will be slower and the energy produced by respiration shall decrease. Preliminary investigation ------------------------- In the first incidence I decided that I would fill a beaker full of 200 cm³ of water from the kettle. The temperature of the water was 70ºC. I noticed at this temperature nothing happened and I then came to the conclusion that the enzymes in the yeast cell had denatured.... ... middle of paper ... ...bath, so to did the rate of reaction and this agrees with the graph of rate of reaction below. This deduction can be made due to the fact that as the enzymes reach the optimum temperature the rate of reaction is grates although once this range of temperatures had been exceeded (from around 35ºC to 45ºC) the rate of reaction has a rapid decline until around the enzymes then become denature which is around 60ºC. Results Table Temperature of water Bath in ºC Minutes taken in 1st Experiment Minutes taken in Experiment Minutes taken in Experiment Average time taken Experiments 55 25 22 27 25 45 9 12 13 11 37 4 6 7 6 29 17 15 20 17 19 29 30 29 29

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