Introduction:
Euglenas are unicellular organisms classified into the Kingdom Protista, and the Phylum Euglenophyta. All euglena have chloroplasts and can make their own food by photosynthesis. They are not completely autotrophic though, euglena can also absorb food from their environment; euglenas usually live in quiet ponds or puddles. (Biologycorner.com, 2016) Euglena is a interesting specie because they are combination of plant and animal. They can feed themselves through photosynthesis like a plant. Also, they move like animal. People have argued for many years about which Kingdom to put them. Most scientists put them in the protest Kingdom. So the question is that how does tempura affect Euglena growth. Personally, if the there is higher
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Add 100 mL of growth media from the prepared stock solution to bioreactor (300-500mL plastic bottle).
2. Add 5 mL of Euglena to bioreactor.
3. Cover your bioreactor with a lid or stopper and gently invert to mix the solutions. Remove the lid or stopper after the solutions have mixed.
4. Measure the initial absorption of your algae sample in a spectrophotometer (ensure the wavelength is set to 370nm).
5. Label the bioreactors according to the temperature.
6. Plug your bioreactor with cotton wool.
7. Place in refrigerator.
8. Repeat steps 1-6 with another bioreactor.
9. Repeat steps 1-8 with the other temperatures.
10. Measure the absorption of your sample 2-3 times per week for a period of 2 weeks. Remember to gently invert your solution each time before taking your
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Through standard curve, we know the relationship between absorbance and cell concentration is that the more absorbance the higher cell concentration. The data was measured by spectrophotometer which can be used to measure the concentration of a known chemical substance. The higher value of dada means that the higher concentration. At the same time, it means that the higher rate of growth. The table and graph directly reflect the relationship between temperature and speed of growth of Euglenas. The graph has shown a trend that first rise after falling. The sharpest change belong room temperature and the gentle change is that when Bioreactors in fridge. Therefore, we can draw a conclusion that when in room temperature (about 23) there is the fastest speed of growth. Excessive temperature will affect the growth of
The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.
coli cells were grown at 37 degrees Celsius with calcium ions. These cells were then incubated in ice and placed into 4 polypropylene tubes. Each tube consisted of 25 microliters of E.coli cells. Two of the four were labeled with (+) while the other two were labeled (-). Positive was with samples that contained 100 pg/ microliter of pGLO, while negative was for the samples that did not. The 4 tubes were incubated in ice for 30 minutes, then heat shocked for 30 seconds at 42 degrees Celsius. The 4 tubes were then returned to the ice bath for 5 minutes. After the removal, .975 microliters of LB media was added to each tube at room temperature. The tubes were then shook at 225 RPM at 37 degrees Celsius for one hour. 300 microliters of cells were then extracted from the tubes and then placed in agar plates. The cells were spread across the plate and the incubated overnight at 37 degrees
The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter, Equipments: Iodine solution: used to test for present of starch - Amylase solution - 1% starch solution - 1 pipette - 3 syringes - 8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter Method: = == ==
...or the dark reaction and the brake for aerobic respiration. This allows for many ATPs to be produced, allowing the kinetosomes to anchor the flagellum of Mr. Euglena through the cell membrane. This anchor enables the flagellum to function, which will move Mr. Euglena to a less crowded area in order to live.
Lillia Davidson, my grandma was born mid war, 1940 to Shirley and Ed Kittle. Lillia, her 2 siblings and their parents lived in a small apartment in Glenbrook, Connecticut from 1940 to 1943, her family moved to the Vermont countryside when she was four. Her parents were not rich Shirley was a seamstress and Ed did was was called a civilian job meaning he did not get paid, he would keep an eye out for suspicious plains. Though her dad was not in the war she felt proud that he was helping with the war. Ed’s job of being a spotter become very important after the bombing of Pearl Harbor in 1941. My grandma has no memory of Pearl Harbor because she was so young. Being so young sheltered her from a lot of what was going on at that time.
Albana Gallari is an Albanian Immigrant who moved to the United States on February 16, 1997. Gallari was born in communist Albania on March 4, 1974. Gallari lived under the reign of Communist Dictator Enver Hoxha throughout her early life. Gallari grew up poor with little to eat besides bread and cheese and had almost no material possessions besides 3 pairs of clothes along with 3 tattered dolls. Throughout her early life her mother took care of her alongside her job of working in a government factory and her father was a livestock veterinarian. Gallari faintly remembers going to her friend's house on Sundays to watch cartoons due to her family being too poor to afford a television. After the communist regime fell in 1992, Gallari became a
Garcinia cambogia is recommended highly in weight loss. It offers an excellent plan that can help to block carbs and fat from entering the liver. The benefits of garcinia have been tried and tested on a number of people drawn from around the world. Pure garcinia contains hydroxycitric acid (HCA), an odorless, completely nontoxic and tasteless substance. According to studies, its diet can help with the loss of 25 pounds within 3 months. The benefits of this food include:
Are you suffering from a nasty cold? Are you sniffling your way through the month, wishing for a remedy? If so, you’re probably researching Echinacea tablets and trying to work out whether or not they might help to ease your symptoms.
Could you imagine going to a impressive waterpark with the Fun Zone, Foam Parties, Wild Waves Wave Pool, 9+ thrilling water slides, and a zipline over the water? LanierWorld, located in Margaritville at Lake Lanier Islands, is the perfect place to have a school field trip. This day trip would be a great get-together over the summer, beginning of the school year gathering, or end of the year party. An added plus, is that it is not hours away, but far away enough to feel like you are in a new, neat environment. LanierWorld is the location for a field trip in Georgia because students would experience hours of fun, excitement, and bonding.
Lycaons are world known for being, "werewolves." Not many people have heard the myths that came from Greek mythology. The Mystery behind this is fascinating and unique compared to what Hollywood has made it be. The areas that need to be focused on is the Myths, His only son, and the downfall of his ruling. This is a Greek Mythological king that more people should understand.
The test tubes were labeled. Each of the Erlenmeyer flasks were filed with approximately 225 mL of regular tap water. The two of the aquatic plant Elodea densa was obtained. Each plant was submerged in a solution of 0.5% solution of sodium bicarbonate in a test tube, and approximately 0.5 cm was cut off the stem. A rubber stopper, containing an attached pipette, and syringe and need was placed over each test tube. Then, the stopper was gently pushed into each test tube, making sure no air bubbles were present. Each of the Erlenmeyer flasks containing the test tubes was then placed in front of a lamp. After being calibrated in front of the lamp for 10 minutes, the reading was taken for 40 minutes. Finally the lamp was turned off. Following the experiment, the results were entered into an Excel spreadsheet. Using the online t-test software “graphpad”, an unpaired t-test was generated. In this investigation the light intensity, and temperature, and aquatic plant (Elodea densa) were constant, and thus where the control variables. (Rush,
The cultures were maintained at 25±20C under 16 hr illumination of 4000 lux intensity. The results are presented in Table 1, it can be seen from the data that pH of the medium had significant effect not only on regeneration frequency but also on number of shoots developed in each culture. Maximum 62.5±4.7 percent cultures in CoS 98259 and 67.3±4.9 percent in CoS 767 developed shoots at pH 6.0 while regeneration frequency was the lowest at pH 5.6. An increase in pH form 6.0 to 6.2 and 6.4 reduced the frequency of shoot regeneration from the callus (Table
Although the experiment produced varying results amongst the pairs of test tubes in each of the water temperatures, the Mean calculations proves that the temperature rising will increase the amount of kinetic energy in the movement of the Phosphate and Lipids in the cell membrane as well as breaking the hydrogen bonds of the proteins in the cell membrane,
Diatoms have a unique characteristic. They live in an outer cell wall, or a frustrule made of silica. This frustrule is not just for the physical appearance, it provides protection and even structure. On the cell wall, there are spikes, spines and pores which allow diatoms to be classified into different classes, the centric (Centrobacillariophyceae) and the pennate (...
Increases in temperature can decrease the efficiency of photosynthesis; however the extent of this impact may be determine on the species' dependency on light.