Disadvantages Of Electron Microscope

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The earliest practices for examining physical evidence in crime laboratories depended merely on the microscope to study the structure and composition of matter. In the early 1950s, the scanning electron microscope (SEM) was developed and it added a new element to forensic science, which was previously unattainable within the restrictions of the ordinary light microscope. All other traditional microscopes use light coming off the specimen to produce a magnified image (Saferstein, 2011). The SEM is a special microscope in the sense that it uses electrons instead of light to produce an image. This type of microscope has allowed forensic scientists to examine a much larger variety of specimens (Scanning Electron Microscope, n.d.).
The scanning electron microscope forms an image by aiming a beam of electrons onto the specimen and observing electron emissions on a closed TV circuit (Saferstein, 2011). There is an electron beam that follows a steep passage through the microscope, which is retained inside of a vacuum. The beam flows through electromagnetic fields and lenses. This allows the beam to focus down toward the sample. Once the sample is hit by the beam, electrons and X-rays are ejected from the sample.
The SEM has many advantages over the older microscopes. One advantage is its large depth of field, which allows more of the chosen sample to be in the focus at the same time. Another advantage is that it has much higher resolution. Therefore, samples that are spaced closely together can be magnified at much higher levels. This microscope also gives the examiner much more control in the degree of magnification because the SEM uses electromagnets rather than lenses (Scanning Electron Microscope, n.d.). Another unique ad...

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...ires that the evidence is not altered during the examination so that it can be later reexamined in its initial status by another examiner. There are two kinds of SEM that are valuable for these issues, which include environmental (ESEM) and low vacuum (LVSEM). Both instruments are operated differently; however, they both allow examination without any preparation that would modify the sample. Validation means that it can be demonstrated in court that the microscope was in working order and the methods used while in operation were acceptable (Kruesemann, 2008). The SEM has many additional uses including examining bullets and studying tool marks made by firing pin impressions. This information will allow forensic scientists to determine the kind of gun that was used. The SEM has proved to be an excellent device for forensic investigations (Firearms Tutorial, n.d.)

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