A microbiome is the proportion and types of microorganisms that are living in a specific location. It is important, for the microbiome not only breaks down in the gut but also absorbs nutrients. They correlate to a person’s health overall, their diet, and their environment. The transformation of bile and the production of amino acids and vitamins are found by bacteria inside the animal gut microbiome. Furthermore, it gives a passage for carbohydrates that are non-digestible as well as allow energy recovery and nutrient for growth in bacteria.
My question for the gut microbiome experiment is, “Will there be a difference in the gut microbiome due to a specific difference between diets among dogs?” My hypothesis for the gut microbiome experiment
is, “If the dogs have a different diet, then there will be a difference in the gut microbiome.” I used samples 307, 455, 459, 510, and 671, which are all dogs. They are all males with good overall health. Their age ranges from six months to ten years old. They can live either indoor or outdoor, and they can live with or without animals. The DNA extraction from the scat was successful in terms of quantity except for Sample 307. Sample 307 was removed since the concentration is lower than the negative control, which indicates cross-contamination occurred. The positive control had the highest concentration, readings, and quantification since the DNA is purified with E. coli. The negative control had the lowest concentration, readings, and quantification, for deionized water was used instead of DNA. Overall, samples 455, 459, 510, and 671 were adequate for sequencing, had high readings, and good quantification. My hypothesis is supported, for there is a difference in the gut microbiome. All of the dogs have different diet. Furthermore, the pie chart displays the bacteria in the dog. Some of the dogs have the same bacteria as the other dogs; however, there are different amounts of that bacteria. Thus, supports my hypothesis that there is a difference in the gut microbiome of dogs having different diets.
...indole, it is motile, there is no urease present and there is no coagulase activity. By deduction and logical reasoning Unknown 10a was determined to be Escherichia coli.
After reading both articles, the “cookie monster” article lacks essential information need for the research. For a research to be experimental there are certain variables that needs to be taken into consideration before arriving at a conclusion. Necessary information needed to understand the experimental ...
...standing the nature of relationship between the residing microbes inside human cells and about their function is very important to put an end to this war and to live in peace with the natural organisms that are benefitting human body and their survival has become our primary importance.
To understand the human gut health and aetiology, the first step is to understand the gastrointestinal (GI) microflora and its distribution through the digestive system [2]. The human GI tract is inhabited by trillions of microorganisms, which together is known as the microbiota [5]. These microorganisms come from both archeal and bacterial domains. Bacteria are the predominant kingdom of organisms and it is composed mainly by five bacterial phyla: Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia [3]. The great majority of mammalian gut microbiota belongs to the three phyla: the Gram-negative anaerobe Bacteroidetes, the Gram-positive Actinobacteria and Firmicutes [5].
Diabetes is a metabolic disease, that causes a person to have high blood pressure. There are two kinds of diabetes, type 1 and type 2. Type 1 is the loss of insulin producing cells, leading to a deficiency of insulin. Type 2 diabetes is where the person is insulin resistant. Insulin is a peptide hormone, which causes cells to absorb the various glucose it requires. Type 2 diabetes is the most widespread endocrine disease worldwide. Metagenome wide associated study took on a study that was based on the determination of type 2 diabetes and relationship to gut microbial by using the deep shot gun sequencing of the gut microbial. The study consisted of 345 Chinese's individuals that were used to help find any relationships. 60000 type 2 diabetes associated markers were used and linked to type 2 diabetes. Gut microbes are living organisms that live in the gut and the digestive tract. Patients with type 2 diabetes were characterized by a moderate degree of gut microbial symbiosis. Symbiosis is a interaction between different microbial organisms, there are various types of symbiosis such as mutalistic, parasitic and communalistic. The abundance of butyrate producing bacteria as well as the opportunistic pathogens were determined as well. Enrichment of microbial functions due to sulphate reduction and oxidative stress resistance was found. Further studies were also performed with addition of individuals to the study. Gut microbial markers may be useful for classifying type 2 diabetes.
Turnbaugh, P. J., Backhed, F., Fulton, L., & Gordon, J. I.. “Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.” Cell Host and Microbe (2008). 3, 213–223.
Should humans test on only certain animals, different animals, or not at all? Many people have different opinions on this topic. In this essay I will argue for the side of not using them for testing while stating defending arguments.
From these experiment many questions and future studies are brought to the table. Some questions about the experiment may include the reliability of the test suspects. Due to the different lifestyles and conditions of each test subject it could have and affect on the results of the experiment. Also the article states that there could have been a mistake in the values of PD__AMP. A future study similar to this may be hookworm’s effect on other disease like issues in the immune system. It would relate to the past two studies because in both of the previously done studies it tested the hookworm’s effect on issues with the immune system. The future studies could be improved by making the test suspects in the most similar conditions and lifestyles as possible. Also they could use a larger number of test subjects to have more accurate results.
3. This freshly isolated microorganism, when inoculated into a healthy laboratory animal, should cause the same disease seen in the original animal.
Throughout centuries medical research has been conducted on animals. “Animals were used in early studies to discover how blood circulates through the body, the effect of anesthesia, and the relationship between bacteria and disease” (AMA 59). Experiments such as these seem to be outdated and actually are by today’s means, scientists now study commonly for three general purposes: (1) biomedical and behavioral research, (2) education, (3) drug and product testing (AMA 60). These three types of experiments allow scientists to gain vast amounts of knowledge about human b...
Microbes are major key components in both are homes and industrial food preparation. There are number of lactic acid which is a form of bacteria which is a large group of beneficial bacteria used in certain foods while they are getting prepared such as yogurt, cheese, sour cream, butter milk and other type of fermented milk products. Things such as vinegars are produced by bacterial acetic acid fermentation. Yeast is also major use in the making of beer and wine and also for the leaving of breads. This also involves fermentations to convert corn and other vegetable carbohydrates to also make beer, wine or gasohol but also bacteria is the agents of are other foods. Other fermented foods will include things such as soy sauce, olives and cocoa. (Microbes and human life, 2013) Single cell proteins are known as dried cells of microbes which are used in protein supplement shacks. They are also called “novel food” and “minifood”. The production of this requires micro-organisms which then serve as the protein source and then the substrate which is biomass which they grow on them. There are a number of both these sources that we are able to use for the production of single cell protein (SCP). The micro-organisms used belong to the following groups of Algae, Fungi and bacteria. (Slide Share, 2012)
Digestive Health: dogs with sensitive stomachs will appreciate fiber and probiotics found in many treats. Prebiotics are found in vegetable fiber, probiotics are live bacteria and both feed the good bacteria in the dog's intestines. This helps your pet to enjoy a better gastrointestinal health and a stronger immune system.
Microbes are microscopic life forms, usually too small to be seen by the naked eye. Although many microbes are single-celled, there are also numerous multi-cellular organisms. The human body has 10-100 trillion microbes living on it, making it one giant super-organism. Since the first link between microbes and diseases was made, people have been advised to wash their hands. Scientists, however, have recently started to investigate more closely how the microbes that call the human body home affect our health. While some microbes cause disease, others are more beneficial, working with our bodies in many subtle ways.
The researchers used DNA sequencing from a male chimpanzee’s blood to present the genomes; it is most closely aligned with humans. This event can explain farther how feasible the animal testing is, and explain the reaction of using animals on testing is just related the DNA sequencing, not about the environment (Lovgren 2005). There are no absolutely things on experiment. Scientists use different animal model and repeated experiments to get surest data that is the best way to test a new product before put it in
Our case is that if we don’t test on animals then progress in scientific fields would be halted. As first speaker for the negative I will speak about the benefits of animal testing in general and then I’ll talk in detail about animal testing in medicine. My second speaker will talk about the opinions on testing and the food chain and my third speaker will summarise our points and rebut.