Unfortunately, most of the waterborne disease outbreaks are the product of failures and system deficiencies (Craun & Calderon, 2001). Today, the water filtration system has greater prevention capacities which decrease the chances of outbreaks (Burlage, 2012). However, accidents happen all the time. One of the most notorious biological contaminants in waterborne disease is Cryptosporidium, which is responsible for one of an essential waterborne outbreak in US soil (Burlage, 2012). According to Bridge et al. (2010), waterborne diseases that derive from microbes are related to environmental processes and can be identified by establishing the concentration of the pathogen in the surface, ground and coastal waters. Microbial contamination is the
What do bacteria need to grow? For bacteria to grow the most typical thing that they like ate a warm and moist environment, but that is not all that they like. Bacteria also like and environment with a PH that is normal or close to a human PH and bacteria also like an oxygen rich environment. The places that could be common to find bacteria in a building are a keyboard, a water fountain, and restrooms. A keyboard is a common place for bacteria because it is being touched constantly with hands when people type and hands are warm, so bacteria like them. The water fountain is another place that is common for bacteria to grow because people's warm hands are touching it and also it has water, which causes it to be moist. The last place that bacteria will we commonly found in buildings are restrooms. The bacteria like restrooms because many people are in then and also there is a lot of water in them.
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
ABSTRACT: Water samples from local ponds and lakes and snow runoff were collected and tested for coliform as well as Escherichia coli. Humans as well as animals come into contact with these areas, some are used for recreational activities such as swimming and some are a source of drinking water for both animals and humans The main goal of this experiment was to see which lakes, snow run off and ponds tested positive for coliform or Escherichia coli and to come up with some reasoning as to why. It was found that the more remote pond with less contact contained the most Escherichia coli. However, another lake that many swim in and use as their drinking water indeed tested positive for a small amount of Escherichia coli. The two samples from the snow showed negative results for both coliform and Escherichia coli and the two more public ponds that aren’t as commonly used as a source of human drinking water but animal drinking water tested in the higher range for coliforms but in the little to no Escherichia coli range. It was concluded that the remote pond should be avoided as it’s not a safe source of drinking water for humans or animals. Other than that, the the other ponds are likely to be safe from Escherichia coli, but coliforms are a risk factor.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
I was given unknown organism #14, in order to find out what organism I had, I had to perform several different biochemical tests to identify it. Starting with the Gram stain test, which is performed to differentiate Gram-positive and Gram-negative cells. After staining, when observed through the microscope Gram-positive cells are a purple color with thick peptidoglycan cell walls. Gram-negative cells are a pinkish/red color with thinner cell walls. (handout G. s.) My organism was observed to be pinkish rod shaped meaning it is Gram-negative bacteria.
Eukaryotic microorganisms can be defined as organisms whose cells contain a nucleus and other organelles enclosed in membranes. It is said that all multicellular organisms are eukaryotes which mostly comprises of animals, plants, and fungi. They are known to be much larger than prokaryotes which contain no nucleus because they are multinucleated organisms. Eukaryotes were said to have developed about 1.6 – 2.1 billion years ago. But that is only an approximation. We would not be here if eukaryotic microorganisms did not exist. These organisms tend to share a common origin and could be treated as a super kingdom, empire, or domain. Lastly, many unicellular organisms are also eukaryotes e.g protozoa. The types of eukaryotes are algae, yeast, fungi and protozoan.
Viruses in the ocean, as products of human activities, are potentially dangerous contaminants of many coastal waters. Total coliform bacteria are a collection of relatively harmless microorganisms that live in large numbers in the intestines of man and warm- and cold-blooded animals. They aid in the digestion of food. A specific subgroup of this collection is the fecal coliform bacteria, the most common member being Escherichia coli or E.Coli. These organisms may be separated from the total coliform group by their ability to grow at elevated temperatures and are associated only with the fecal material or warm-blooded animals. Fecal Coliform is the bacterium that forms in water from fece of humans and other warm-blooded animals. These types of feces can enter directly by animals, such as birds, by discharging into river. Feces also enter water by agricultural and storm run off carrying animal waste, and human sewage discharge.(www.stale.ky.us) The presence of fecal coliform bacteria in aquatic environments indicates that the water has been contaminated with the fecal material of man or other animals. Fecal coliform is not dangerous itself. Its the pathogens that may be found along with fecal coliform that is harmful. Human pathogenic viruses are not only found in the millions of gallons of variously treated human wastes dumped directly into coastal waters from sewage outfalls, but also from runoff from numberous storm drain sewers. Fecal coliform measures are important because they can help determine the probability of contracting a disease from the water. So, the more fecal coliform, the higher the probability of a disease causing bacteria being found in the water.
...nvironmental Microbiology. New York: A John Wiley & Sons, Inc; 1992. pp. 125?156. Accessed December 2, 2013.
In the last decade, the number of prescriptions for antibiotics has increases. Even though, antibiotics are helpful, an excess amount of antibiotics can be dangerous. Quite often antibiotics are wrongly prescribed to cure viruses when they are meant to target bacteria. Antibiotics are a type of medicine that is prone to kill microorganisms, or bacteria. By examining the PBS documentary Hunting the Nightmare Bacteria and the article “U.S. government taps GlaxoSmithKline for New Antibiotics” by Ben Hirschler as well as a few other articles can help depict the problem that is of doctors prescribing antibiotics wrongly or excessively, which can led to becoming harmful to the body.
Extensive studies on various factors affecting Giardia muriscyst inactiva-tion by ozone are reported. These encompass factors like temperature, turbidity, pH, ozone dose, and contact time. It was found that residual and utilised ozone both had important influences in G. muriscyst inactivation. Encephalitozoon intestinalis, a microsporidian pathogen for humans and animals that is detected in surface water, has been listed as a major potential emerging waterborne pathogen by the USEPA. Ozone has been very effective in rendering these spores inactive, and its CXT values were an order of magnitude lower than the CXT values of chlorine in a comparative study (John et al., 2005).Ozone has several advantages, such as being one of the strongest oxidants, not producing chlorinated THMs or HAAs, and being effective against Cryptosporidium, to name
Bacterial cells, like plant cells, are surrounded by a cell wall. However, bacterial cell walls are made up of polysaccharide chains linked to amino acids, while plant cell walls are made up of cellulose, which contains no amino acids. Many bacteria secrete a slimy capsule around the outside of the cell wall. The capsule provides additional protection for the cell. Many of the bacteria that cause diseases in animals are surrounded by a capsule. The capsule prevents the white blood cells and antibodies from destroying the invading bacterium. Inside the capsule and the cell wall is the cell membrane. In aerobic bacteria, the reactions of cellular respiration take place on fingerlike infoldings of the cell membrane. Ribosomes are scattered throughout the cytoplasm, and the DNA is generally found in the center of the cell. Many bacilli and spirilla have flagella, which are used for locomotion in water. A few types of bacteria that lack flagella move by gliding on a surface. However, the mechanism of this gliding motion is unknown. Most bacteria are aerobic, they require free oxygen to carry on cellular respiration. Some bacteria, called facultatibe anaerobes can live in either the presence or absence of free oxygen. They obtain energy either by aerobic respiration when oxygen is present or by fermentation when oxygen is absent. Still other bacteria cannot live in the presence of oxygen. These are called obligate anaerobes. Such bacteria obtain energy only fermentation. Through fermentation, different groups of bacteria produce a wide variety of organic compounds. Besides ethyl alcohol and lactic acid, bacterial fermentation can produce acetic acid, acetone, butyl alcohol, glycol, butyric acid, propionic acid, and methane, the main component of natural gas. Most bacteria are heterotrophic bacteria are either saprophytes or parasites. Saprophytes feed on the remains of dead plants and animals, and ordinarily do not cause disease. They release digestive enzymes onto the organic matter. The enzymes breakdown the large food molecules into smaller molecules, which are absorbed by the bacterial cells. Parasites live on or in living organisms, and may cause disease. A few types of bacteria are Autotrophic, they can synthesize the organic nutrients they require from inorganic substances. Autotrophic bacteria are either photosynthetic or Chemosynthetic. The photosynthetic bacteria contain chlorophyll that are different from the plant chlorophyll. In bacterial photosynthesis, hydrogen is obtained by the splitting of compounds other than water.
“Recreational water illnesses (RWIs) are caused by germs spread by swallowing, breathing in mists or aerosols of, or having contact with contaminated water in swimming pools, hot tubs, water parks, water play areas, interactive fountains, lakes, rivers, or oceans. RWIs can also be caused by chemicals in the water or chemicals that evaporate from the water and cause indoor air quality problems. RWIs may include a wide variety of infections, including gastrointestinal, skin, ear, respiratory, eye, neurologic and wound infections. Many RWIs (skin, ear, eye, respiratory, neurologic, wound, and other infections) are caused by germs that live naturally in the environment (for example, in water and soil)” (Centers for Disease Control and Prevention).
Water-borne transmission refers to diseases that are acquired via usually contaminated freshwater. Infection occurs during bathing, swimming, drinking, preparing of food, or in the consumption of food that has been infected with a water-borne pathogen. Most of water-borne pathogens are protozoa, bacteria, viruses, and parasites. Common examples of water-borne pathogens are Cryptosporidium parvum, Giardia lamblia, Campylobacter jejuni, Vibrio cholerae, and Legionella pneumophila, along with man...
Have you ever had to walk miles away just to get clean drinking water, or don’t even not have access to clean drinking water? People all over the world, even in North America, don’t have access to clean drinking water or have to walk very far just to drink water. The main areas where this problem is prominent is in third world countries, and this is due to the lack of money and sanitation (Millions Lack Safe Water). Due to this lack of sanitation, water borne diseases can grow and infect people who consume it. Clean water is very important for life, and within this paper I will explain why we need it, how it can affect us, and what it will take to obtain clean water.
Knowledge is power when discussing the classes you have taken in college and how it affects your everyday life. Microbiology is one of those important classes where although for a majority of majors you do not have to take it and therefore will not learn the information discussed in it, other majors such as biology and nursing require you to take it. They will require you to take it because you will encounter some of the information being taught in it in the work field. As a nurse practitioner I have worked in the field with many patients who were diagnosed with Leprosy, Escherichia coli, Salmonella and other bacterial diseases. All of these diseases that I have encountered in patients were ones I was familiar with due to learning about them as a student in microbiology at CSUB. My children plan on