Title: Preparation of thin blood smear.
Aim and objective: To produce blood smear and stain by using the wrights stain
Introduction:
A blood smear is a diagnostic test used to look for unusualness within the blood. The blood film gifts the opportunity to view the components of blood under a microscope for unusual shapes or sizes. There are three main cells within the blood that the test can focus such as red blood cell, white blood cells, and platelets.
Red blood cell or erythrocytes carry
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Using your right hand, the smooth clean edge of a spreader on the specimen slide is placed in front of the blood drop.
4. The spreader slide is held at a 30°, and the drop of blood is drawn back
5. The blood is spread almost to the edges
6. The spread is pushed forward with one light, smooth, and fluid motion. A thin of blood in the shape of a bullet or tongue with a feathered edge will remain on the slide.
7. The frosted edge is labelled with patient name, identity card, and date.
8. The blood film is allowed to air-dry completely before staining.
Preparation of Thin Blood Smear:
1. A small drop of venous blood is placed on a glass of microscope slide, using a disposable pipette. Used a wooden applicator stick for this purpose
2. A spreader slide is positioned at an angle and drawn slowly toward the drop of blood.
3. Brought the spreader slide in contact with the drop of blood and drawn it away. The edge of the spreader slide is noted.
4. The spreader slide is pulled out further makes a thin layer of blood.
5. The blood smear is nearly completed
6. Result ended. A glass slides with a well-formed blood film. After dried for about 10 minutes, the slide is stained manually or an automated slide stain is placed.
Staining
The procedure of the test is simple hence patients will then be asked to remove their clothes and wrap and cover with a paper cloth around the waist before the Pap test. Patients will then be asked to lie back on the examine table with their legs spread open .Knees have to be bent and feet sitting on rest position. The clinician or gynecologist will insert the lubricated instrument called speculum into the patient’s vagina in a way that will hold open to see the cervix clearly. The clinician will examine to see if there is any visible problem and will gently scrape the surface of the cervix with the help of wooden scraper or spatula to obtain the sample of the cells. The liquid sample will then be smeared on a glass slides and will be brought to a laboratory to analyze where microscope slides will be ready for the test. The clinicians will have to repeat the test if the result is abnormal .Cells abnormalities are divided as follows ASC atypical squamous cells ,AGC,AIS,LSIL.
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The idea is to get a sort of background story from the random blood distribution. The different types of blood can tell us the method an assailant uses, such as passive blood stains. These stains are made from the drops of a weapon due to gravity. For instance, if an attacker walked away from stabbing someone, the weapon would leave a series of drops.... ... middle of paper ...
strips was put into each tube and then timed with a stop clock and the
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dragging of the tissues under the skin. Follow the rule “the deeper you go, the slower you go”.
· A sterile cloth is then put over your legs and abdomen and a sterile
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