Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
digestive system physiology imp qus
digestive system physiology imp qus
digestive system physiology imp qus
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Recommended: digestive system physiology imp qus
Introduction:
The purpose of this lab was to determine the effects that pH had on lactase activity of four different digestive supplements that assist in the breakdown of lactose in the body. Lactase is an enzyme that digests lactose, it is also the primary sugar found in milk (BIOB 260 Lab Manual, 2014). Before it can be broken down lactose needs to be broken down into glucose and galactose for easy absorption into the bloodstream, this is done by the hydrolying of the monosaccharide (BIOB 260 Lab Manual, 2014). Lactase has the ability to catalyze reactions to allow for proper function of breaking down the lactose(BIOB 260 Lab Manual, 2014). To ensure proper function of the lactase enzyme, optimal temperature and pH are needed. Without these conditions the enzyme becomes denatured and lose their functionality (BIOB 260 Lab Manual, 2014). Denature can be defined as destroy the characteristic properties of (a protein or other biological macromolecule) by heat, acidity, or other effects that disrupt its molecular conformation (dictionary.com). pH can be defined as the measure of hydrogen ion concentration of an aqueous solution (BIOB 260 Lab Manual, 2014). pH can be maintained by buffers which accepting protons when concentration rises or donating protons when concentration falls as needed(BIOB 260 Lab Manual, 2014).
Equilibrium between the undissociated and dissociated for of acid in water:
- undissociated acid <---> ions of dissociated acid
- CH3COOH <---> CH3COO- + H+
Reaction showing change of the pH solution:
- CH3COONa <---> CH3COO- + Na+
The hypothesis of the lab was: Digestive Advantage will be the least effective at breaking down lactose than the other three digestive supplements (Fast Act, Up & Up, and Equate) te...
... middle of paper ...
...ssa. Lactase - A Necessary Enzyme Found in Living Organisms. N.d. Photograph. Molecular Anatomy ProjectWeb. 3 Feb 2014. .
"Dictionary.com." Dictionary.com. Lexico Publishing, n.d. Web. 4 Feb 2014. .
Freeman, Scott. Biological Science (BIOB 260 Lab Manual). 4th ed. Print.
Lactose intolerance and health disparities among African Americans and Hispanic Americans: an updated consensus statement.. Diss. Department of Psychiatry at Meharry Medical College, 2013. Web. .
Ojetti, Veronica. Lactose intolerance: from diagnosis to correct management.. Diss. Catholic University of the Sacred Heart, Rome, Italy: Department of Internal Medicine and Gastroenterology, 2013. Web. .
The affects of pH, temperature, and salt concentration on the enzyme lactase were all expected to have an effect on enzymatic activity, compared to an untreated 25oC control. The reactions incubated at 37oC were hypothesized to increase the enzymatic activity, because it is normal human body temperature. This hypothesis was supported by the results. The reaction incubated to 60oC was expected to decrease the enzymatic activity, because it is much higher than normal body temperature, however this hypothesis was not supported. When incubated to 0oC, the reaction rate was hypothesized to decrease, and according to the results the hypothesis was supported. Both in low and high pH, the reaction rate was hypothesized to decrease, which was also supported by the results. Lastly, the reaction rate was hypothesized to decrease in a higher salt concentration, which was also supported by the results.
For example, if a person had been able to consume lactose products for their life with no problems, but in an unfortunate event had to have a portion of his or her small intestine removed, there would be a change in the number of present lactase enzymes in the stomach. Because the lactase enzyme is stored in the small intestine, the person may now experience lactose intolerance due to the decrease in the presence of lactase. Knowing where the lactase enzyme is stored can aid physicians in understanding what will happen after a procedure or the introduction of a new medication. The experiment was conducted to determine the optimal ph of lactose required to produce the maximum amount of glucose. It was predicted that the optimal ph of lactose would be most efficient at lactose ph 6, and that the lower the ph, the amount of glucose produced would increase
While the tube for specimen Cb turned a tannish white in the lower half of the tube while the top stayed the lavender inoculated tube color. Do to this evidence I determined that both specimens Ca and Cb cannot use the process Casein hydrolysis or Casein coagulation due to lack of soft or firm curds in both tubes. Since there was no casein curds formed, I concluded that specimens Ca and Cb also cannot perform the process of proteolysis. My conclusion is supported by the fact that there was no clearing of the medium. I have also determine that neither of my organisms can make the enzymes rennin, proteolytic or even proteases. I know my specimens cannot produce proteases due to the fact that there was no blue coloring in the tubes which means that the byproduct Ammonia was not produced to increase the pH. Since neither of my specimens can make these enzymes, I concluded that my specimens cannot break down lactose or casein. Although I did learn that specimen Cb can reduce litmus due to the evidence that the lower part of the tube turned a tannish white color with a purple ring at the top. This color change from a purple to a white means that the litmus was reduced turning it clear and leaving the white of the milk to show. Finally I know that specimen Ca cannot reduce litmus due to the fact that the tube had no change in
Lactase is an enzyme found in the digestive system. It is essential to the complete digestion of sugar in whole milk and milk products. Lactase specifically breaks down lactose, a complex sugar. Lactase cannot be absorbed by the body unless it is broken down by lactase into glucose and galactose. According to webMD, “Lacking lactase in their intestines, a person consuming dairy products may experience the symptoms of lactose intolerance…Abdominal cramping, flatulence (gas) and diarrhea can occur when a lactose intolerant person consumes milk products.” ("Lactase Enzyme oral : Uses, Side Effects, Interactions, Pictures, Warnings & Dosing - WebMD", n.d.) Lactase is not recommended for use in CHILDREN younger than 4 years of age. Safety and effectiveness in this age group have not been confirmed. (Kluwer, 2014)
LI was first recognized in the 1960s when researchers found black children responding unfavorably to milk in their diets (Harrison 812). Research led to the discovery that lactose, the major sugar in milk and related dairy products, was undigestible in some people because they were missing the enzyme lactase. Lactase breaks down lactose into its component monosaccharide sugars, glucose and galactose. In people missing lactase, lactose passes undigested through the small intestine. In some people, the undigested lactose passes through the remainder of their systems with no ill effects. In others, however, the undigested lactose becomes viscous and ferments in the colon (Englert and Guillory 903). The thickness of the liquid and the fermentation cause painful cramping, gas and sometimes diarrhea. Besides not being able to digest lactose, these people suffer from malabsorption, which causes them to receive little or none of milk's nutrients (Houts 110).1
The purpose of this experiment was to determine the effects that varying temperatures, enzyme concentration, and pH had on catalase activity.
•The forty five year old patient is diagnosed with the progressive cirrhosis inflaming the liver along with the parenchymal cells. The plain symptoms is manifested primarily because of the augmentation of edema internally in the lower abdomen.
Digestion is defined as the process of transforming foods into unites for absorption. The Digestive System is a complex network of organisms that have six major processes: The digestion of food, the secretion of fluids and digestive enzymes, the mixing and movement of food and waste throughout the body, the digestion of food into smaller pieces, the absorption of nutrients, and the excretion of wastes (Inner Body (1).)
Provide muscle energy, fuel for the nerve system, the fat metabolism and protect the protein to been used as energy
The purpose of this experiment is to detect what kind of macromolecules are present in these three types of milk by using the Benedict’s solution, Lugol’s solution, and Sudan IV solution. Also, using the nutrition facts labels to identify which substance is skim milk, whole milk, and soy milk. Hypothesis: Using the Benedict’s solution to detect for the presence of simple sugar. If the unknown A, B, C milk samples turn from bright blue to orange color during the Benedict's test, then these samples are positive control and the carbohydrates are present in them.
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
Lactase, a type of enzyme usually found in the small intestine, breaks down lactose into sugars such as galactose and glucose. People that are lactose intolerant cannot consume anything containing dairy because they cannot break down lactose, a sugar found in milk. Those that are lactose intolerant lack the enzyme lactase. Without lactase, the body does not have the ability to break down lactose, which leads to a person having an upset stomach and diarrhea. Adults are more likely to be lactose intolerant than children because of the metabolic change in the body (Dritsas). The lack of lactase that people have can be compensated by taking pills to help break down lactose that is consumed; with the help of a lactase pill the body can now absorb galactase and glucose properly (McCracken, 481).
The human digestion system is very complex. It starts with the mouth, salivary glands, pharynx, esophagus, stomach, liver, pancreas, gallbladder, small intestine, large intestine, then ends/exits with the anus. Each step is essential to the whole system. For example, the mouth chews food and mixes it with saliva produced by the salivary glands, and then the pharynx swallows chewed food mixed with saliva, this is followed by the food traveling through the esophagus to the stomach where the food gets a bath and mixes with acids and enzymes. After the stomach, the liver, pancreas, and gallbladder produce, stores, and releases bile and bicarbonates. Bile is produced in the liver and aids in digestion and absorption of fat while the gallbladder stores bile and releases it into the small intestine when needed. Following the process into the small intestine, this is where nutrients will be absorbed into the blood or lymph (most digestion occurs here). Next is the large intestine this is where water and some vitamins and minerals are absorbed. Finally, it is the end of the road, the anus. At...
... Dietetic Association. Journal of the American Dietetic Association 95.10 (1995): 1160. ProQuest. Web. 3 Dec. 2013.
The digestive system is a very important system in the human body. It is a group of organs that work together to turn food into energy and nutrients in the entire body. The food that was chewed in a humans’ mouth now passes through a long tube that is inside of the body that is known as the alimentary canal. The alimentary canal is made of the oral cavity, pharynx, esophagus, stomach, small intestines, and large intestines. Those few things are not the only important accessories of the digestive system there is also the teeth, tongue, salivary glands, liver, gallbladder, and pancreas.